Mouse anti-Chicken CD8a-PE/CY5 Monoclonal Antibody
Mouse Anti-Chicken CD8a-PE/CY5
Reactivity
Chicken
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD8a-PE/CY5; Monoclonal Antibody; Mouse Anti-Chicken CD8a-PE/CY5; Mouse Anti-Chicken CD8alpha; anti-CD8a-PE/CY5 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
CT-8
Specificity
alpha chain (34-kDa) of chicken CD8
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gammadelta CD8alphabeta cells in the chicken that is not found in rodents.1,3 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,2 MAb CT-8 recognizes the CD8alpha chain.1
In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gammadelta CD8alphabeta cells in the chicken that is not found in rodents.1,3 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,2 MAb CT-8 recognizes the CD8alpha chain.1
Form/Format
Spectral Red (SPRD) Conjugate
Applicable Applications for anti-CD8a-PE/CY5 antibody
Identification and enumeration of CD8+cells by Flow Cytometry 2, Identification of CD8+ T cells in acetone-fixed, Frozen tissue sections4, Immunoprecipitation 2
Application Notes
Flow Cytometry: <= 0.1 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this product insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
References
1. Tregaskes, C.A., F. Kong, E. Paramithiotis, C.-L. Chen, M.J.H. Ratcliffe, T. F. Davison, and J.R. Young. 1995. J. Immunol. 154:4485.
2. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
3. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.
4. Chen, C.H. 1996. Personal communication.
2. Chan, M.L., C.-L. Chen, L. Ager, and M.D. Cooper. 1988. J. Immunol. 140:2133.
3. Cooper, M.D., R.P. Bucy, and C.-L. Chen. 1990. In: The Avian Model in Developmental Biology: From Organism to Genes. Editions du CNRS, pp. 239-249.
4. Chen, C.H. 1996. Personal communication.
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Product Notes
The CD8a-PE/CY5 (Catalog #AAA670297) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken CD8a-PE/CY5 reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD8a-PE/CY5 can be used in a range of immunoassay formats including, but not limited to, Identification and enumeration of CD8+cells by Flow Cytometry 2, Identification of CD8+ T cells in acetone-fixed, Frozen tissue sections4, Immunoprecipitation 2. Flow Cytometry: <= 0.1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD8a-PE/CY5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8a-PE/CY5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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