Mouse anti-Human SMAC/DIABLO Monoclonal Antibody | anti-DIABLO antibody
Mouse Anti-Human SMAC/DIABLO-UNLB
SMAC/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. SMAC/DIABLO has been shown to neutralize the antiapoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis. 1-6
ELISA Suggested Dilution: 1:1,000-1:2,000
Reagents are stable for the period shown on the label if stored as directed.
2. Hu, S., and X. Yang. 2003. J. Biol. Chem. 278:10055.
3. Du, C., M. Fang, Y. Li, L. Li, and X. Wang. 2000. Cell 102:33.
4. Adrain, C., E.M. Creagh and S.J. Martin. 2001. EMBO. 20:6627.
5. Srinivasula, S.M., P.Datta, X. Fan, T. Fernandes-Alnemri, Z. Huang, and E.S. Alnemri. 2000. J. Biol. Chem. 275:36152.
6. Ekert, P.G., J. Silke, C.J. Hawkins, A.M. Verhagen, and D.L. Vaux. 2001. J. Cell Biol. 152:483.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq]
Uniprot Description
DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Mitochondrial
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol
Molecular Function: protein binding
Biological Process: caspase activation; neuron apoptosis; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress
Disease: Deafness, Autosomal Dominant 64
Research Articles on DIABLO
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Product Notes
The DIABLO diablo (Catalog #AAA670144) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human SMAC/DIABLO-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAC/DIABLO can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Western Blot Suggested Dilution: 1:5,000-1:10,000 ELISA Suggested Dilution: 1:1,000-1:2,000. Researchers should empirically determine the suitability of the DIABLO diablo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAC/DIABLO, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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