Rat anti-Mouse IL-17 Monoclonal Antibody | anti-IL17A antibody
Rat Anti-Mouse IL-17-LE/AF
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Western Blot
Synonyms
IL-17; Monoclonal Antibody; Rat Anti-Mouse IL-17-LE/AF; Rat Anti-Mouse IL-17 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-17; anti-IL17A antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
TC11-18H10
Specificity
Mouse Interleukin-17 (IL-17)
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Applicable Applications for anti-IL17A antibody
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
Application Notes
Research Application(s): ELISA Capture: TC11-18H10 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-17 protein levels. Purified TC11-18H10 antibody should be paired with biotinylated TC11-8H4 antibody as the detection antibody, with purified recombinant mouse IL-17 as the standard.
Immunofluorescence/Flow Cytometry: TC11-18H10 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-17-positive cells within mixed cell populations. Rat IgG1 should be used as a specificity control. Western Blotting: The purified TC11-18H10 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-17 under reducing conditions.
Neutralization: The LE/AF form of TC11-18H10 can be used for neutralizing mouse IL-17 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunofluorescence/Flow Cytometry: TC11-18H10 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-17-positive cells within mixed cell populations. Rat IgG1 should be used as a specificity control. Western Blotting: The purified TC11-18H10 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-17 under reducing conditions.
Neutralization: The LE/AF form of TC11-18H10 can be used for neutralizing mouse IL-17 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
E. coli-expressed recombinant mouse IL-17
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,504 Da
NCBI Official Full Name
IL-17
NCBI Official Synonym Full Names
interleukin 17A
NCBI Official Symbol
IL17A
NCBI Official Synonym Symbols
IL17; CTLA8; IL-17; IL-17A
NCBI Protein Information
interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
UniProt Protein Name
Interleukin-17A
Protein Family
UniProt Gene Name
IL17A
UniProt Synonym Gene Names
CTLA8; IL17; IL-17; IL-17A; CTLA-8
UniProt Entry Name
IL17_HUMAN
NCBI Description
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
Subunit structure: Homodimer. Ref.8
Subcellular location: Secreted.
Tissue specificity: Restricted to activated memory T-cells.
Post-translational modification: Found both in glycosylated and nonglycosylated forms.
Sequence similarities: Belongs to the IL-17 family.
Research Articles on IL17A
Similar Products
Product Notes
The IL17A il17a (Catalog #AAA670091) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-17-LE/AF reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-17 can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunofluorescence; Western Blot. Research Application(s): ELISA Capture: TC11-18H10 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-17 protein levels. Purified TC11-18H10 antibody should be paired with biotinylated TC11-8H4 antibody as the detection antibody, with purified recombinant mouse IL-17 as the standard. Immunofluorescence/Flow Cytometry: TC11-18H10 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-17-positive cells within mixed cell populations. Rat IgG1 should be used as a specificity control. Western Blotting: The purified TC11-18H10 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-17 under reducing conditions. Neutralization: The LE/AF form of TC11-18H10 can be used for neutralizing mouse IL-17 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IL17A il17a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-17, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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