Rat anti-Mouse IL-10 Monoclonal Antibody | anti-IL-10 antibody
Rat Anti-Mouse IL-10-LE/AF
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence
Synonyms
IL-10; Monoclonal Antibody; Rat Anti-Mouse IL-10-LE/AF; Rat Anti-Mouse IL-10 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-10; anti-IL-10 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b kappa
Clone Number
JES5-16E3
Specificity
Mouse Interleukin-10 (IL-10)1, 2 (This is a neutralizing antibody)
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Sequence Length
168
Applicable Applications for anti-IL-10 antibody
ELISA; Flow Cytometry; Immunofluorescence
Application Notes
Research Application(s): ELISA Detection: JES5-16E3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels. Biotinylated JES5-16E3 antibody should be paired with purified JES5-2A5 antibody as the capture antibody, with purified recombinant mouse IL-10 as the standard.
Immunofluorescence/Flow Cytometry: JES6-16E3 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant mouse IL-10, unlabeled or biotinylated Rat IgG2b should be used as a specificity control. FITC conjugated JES5-16E3 antibodies are useful for intracellular staining in cells producing IL-10. our intracellular staining buffers are recommended for this application.
Neutralization: The LE/AF form of JES5-16E3 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunofluorescence/Flow Cytometry: JES6-16E3 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant mouse IL-10, unlabeled or biotinylated Rat IgG2b should be used as a specificity control. FITC conjugated JES5-16E3 antibodies are useful for intracellular staining in cells producing IL-10. our intracellular staining buffers are recommended for this application.
Neutralization: The LE/AF form of JES5-16E3 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
E. coli-expressed recombinant mouse IL-10
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. J. Immunol. Meth. 166:201.
2. Litton, M.J., B. Sander, E. Murphy, and J.S. Abrams. 1994. J. Immunol. Meth. 175:47.
2. Litton, M.J., B. Sander, E. Murphy, and J.S. Abrams. 1994. J. Immunol. Meth. 175:47.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
Similar Products
Product Notes
The IL-10 (Catalog #AAA670083) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-10-LE/AF reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10 can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunofluorescence. Research Application(s): ELISA Detection: JES5-16E3 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels. Biotinylated JES5-16E3 antibody should be paired with purified JES5-2A5 antibody as the capture antibody, with purified recombinant mouse IL-10 as the standard. Immunofluorescence/Flow Cytometry: JES6-16E3 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant mouse IL-10, unlabeled or biotinylated Rat IgG2b should be used as a specificity control. FITC conjugated JES5-16E3 antibodies are useful for intracellular staining in cells producing IL-10. our intracellular staining buffers are recommended for this application. Neutralization: The LE/AF form of JES5-16E3 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IL-10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
