Rat anti-Mouse IL-10 Monoclonal Antibody | anti-IL-10 antibody
Rat Anti-Mouse IL-10-LE/AF
Reactivity
Mouse
Applications
ELISA, Western Blot
Synonyms
IL-10; Monoclonal Antibody; Rat Anti-Mouse IL-10-LE/AF; Rat Anti-Mouse IL-10 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-10; anti-IL-10 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
JES5-2A5
Specificity
Mouse Interleukin-10 (IL-10)1-3 (This is a neutralizing antibody.)
Form/Format
Low Endotoxin, Azide - Free (LE/AF)
Sequence Length
168
Applicable Applications for anti-IL-10 antibody
ELISA; Western Blot
Application Notes
Research Application(s): ELISA Capture/Detection: JES5-2A5 monoclonal antibody is useful as a capture or a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels.1-5 Purified JES5-2A5 antibody can be paired with biotinylated JES5-16E3 antibody as the detection antibody, with purified recombinant mouse IL-10 as the standard.
Western Blotting: The purified JES5-2A5 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
Neutralization: The LE/AF form of JES5-2A5 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Western Blotting: The purified JES5-2A5 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-5 under reducing conditions.
Neutralization: The LE/AF form of JES5-2A5 can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
E. coli-expressed recombinant mouse IL-10
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
4. Mo, X.Y., S. R. Sarawar, and P. Doherty. 1995. Induction of cytokines in mice with parainfluyenza pneumonia. J. Virol. 69:1288-1291.
5. Sarawar, S. M. Sangster, R. Coffman, and P. Doherty. 1994. Administration of anti-IFN- gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper 2 phenotype. J. Immunol. 153:1246-1253.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
4. Mo, X.Y., S. R. Sarawar, and P. Doherty. 1995. Induction of cytokines in mice with parainfluyenza pneumonia. J. Virol. 69:1288-1291.
5. Sarawar, S. M. Sangster, R. Coffman, and P. Doherty. 1994. Administration of anti-IFN- gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper 2 phenotype. J. Immunol. 153:1246-1253.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
