Rat anti-Human G-CSF Monoclonal Antibody | anti-CSF3 antibody

Rat Anti-Human G-CSF-BIOT

Cat. Num. AAA670060

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33; MGC45931
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: G-CSF; Monoclonal Antibody; Rat Anti-Human G-CSF-BIOT; Rat Anti-Human G-CSF - Biotin; Rat Anti-Human G-CSF; anti-CSF3 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: BVD11-37G10
• Specificity: Human Granulocyte-Colony Stimulating Factor (G-CSF)1,2 (This is a neutralizing antibody.)
• Form/Format: Biotin (BIOT) Conjugate
• Sequence Length: 138

• Applicable Applications for anti-CSF3 antibody: ELISA; Western Blot
• Application Notes: Research Application(s): ELISA Detection: BVD11-37G10 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels.1,2 Biotinylated BVD11-37G10 antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human G-CSF as the standard.; Western Blotting: The purified BVD11-37G10 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human G-CSF under reducing conditions.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: E. coli-expressed recombinant human G-CSF
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; Reagents are stable for the period shown on the label if stored as directed.

References

1. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.

NCBI and Uniprot Product Information

• NCBI GI #: 297660573
• NCBI GeneID: 1440
• UniProt Accession #: P09919; Q5FBY7; Q6FH65; Q8N4W3
• Molecular Weight: 22,293 Da
• NCBI Official Full Name: granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33; MGC45931
• NCBI Protein Information: granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; OTTHUMP00000164325; OTTHUMP00000164326; OTTHUMP00000164327; OTTHUMP00000164328
• UniProt Protein Name: Granulocyte colony-stimulating factor
• Protein Family: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Post-translational modification: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Pharmaceutical use: Available under the names Neupogen or Granulokine (Amgen/Roche) and Granocyte (Rhone-Poulenc). Used to treat neutropenia (a disorder characterized by an extremely low number of neutrophils in blood).

Sequence similarities: Belongs to the IL-6 superfamily.

Caution: Ref.5 misquotes the gene name as "CSF1".