Mouse anti-Human IFN- gamma Monoclonal Antibody | anti-IFNG antibody
Mouse Anti-Human IFN-g-FITC
Immunofluorescence/Flow Cytometry: B27 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN- gamma -positive cells within mixed cell populations. Recombinant human IFN- gamma, unlabeled B27, or Rat IgG1 should be used as a specificity control.
Immunoprecipitation: The B27 antibody has been reported to be useful for immunoprecipitation.1
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Reagents are stable for the period shown on the label if stored as directed.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.
Uniprot Description
Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subunit structure: Homodimer.
Subcellular location: Secreted.
Tissue specificity: Released primarily from activated T lymphocytes.
Post-translational modification: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
Involvement in disease: In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [
MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Pharmaceutical use: Available under the name Actimmune (Genentech). Used for reducing the frequency and severity of serious infections associated with chronic granulomatous disease (CGD).
Sequence similarities: Belongs to the type II (or gamma) interferon family.
Research Articles on IFNG
Similar Products
Product Notes
The IFNG ifng (Catalog #AAA670030) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IFN-g-FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFN- gamma can be used in a range of immunoassay formats including, but not limited to, ELISA; Flow Cytometry; Immunofluorescence; Immunoprecipitation. Research Application(s): ELISA Detection: B27 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IFN- gamma protein levels.1,2 Biotinylated B27 antibody should be paired with purified A35 antibody as the capture antibody, with purified recombinant human IFN- gamma as the standard. Immunofluorescence/Flow Cytometry: B27 antibodies are useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IFN- gamma -positive cells within mixed cell populations. Recombinant human IFN- gamma, unlabeled B27, or Rat IgG1 should be used as a specificity control. Immunoprecipitation: The B27 antibody has been reported to be useful for immunoprecipitation.1 Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IFNG ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN- gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
