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Flow Cytometry (FC/FACS) (Fig-1: Cell Surface flow analysis of Anti- hCD13 in PBMC (Granulocytes gated) using 0.2 ug/10^6 cells. Green represents FITC conjugated isotype control; red represents FITC conjugated anti-CD13 antibody (10-4128-F).)

Mouse anti-Human hCD13 Monoclonal Antibody | anti-hCD13 antibody

Monoclonal Antibody to hCD13 (Clone: BF10) FITC Conjugated

Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Purified
Protein G Chromatography
Synonyms
hCD13; Monoclonal Antibody; Monoclonal Antibody to hCD13 (Clone: BF10) FITC Conjugated; Aminopeptidase N; Alanyl aminopeptidase; Aminopeptidase M; Microsomal aminopeptidase; gp150; anti-hCD13 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
BF10
Purity/Purification
Purified
Protein G Chromatography
Form/Format
0.2 mg/ml in Tris buffer containing 0.05% Azide
Sequence Length
967
Applicable Applications for anti-hCD13 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: 0.2-0.5 ug/10^6 cells
Antigen
CD13
Immunogen Information
KG-1a cell line were used as immunogen for this antibody.
Conjugation
FITC
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.

Flow Cytometry (FC/FACS)

(Fig-1: Cell Surface flow analysis of Anti- hCD13 in PBMC (Granulocytes gated) using 0.2 ug/10^6 cells. Green represents FITC conjugated isotype control; red represents FITC conjugated anti-CD13 antibody (10-4128-F).)

Flow Cytometry (FC/FACS) (Fig-1: Cell Surface flow analysis of Anti- hCD13 in PBMC (Granulocytes gated) using 0.2 ug/10^6 cells. Green represents FITC conjugated isotype control; red represents FITC conjugated anti-CD13 antibody (10-4128-F).)
Product Categories/Family for anti-hCD13 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
290
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109,540 Da
NCBI Official Full Name
aminopeptidase N
NCBI Official Synonym Full Names
alanyl aminopeptidase, membrane
NCBI Official Symbol
ANPEP
NCBI Official Synonym Symbols
APN; CD13; LAP1; P150; PEPN; GP150
NCBI Protein Information
aminopeptidase N
UniProt Protein Name
Aminopeptidase N
UniProt Gene Name
ANPEP
UniProt Synonym Gene Names
APN; CD13; PEPN; AP-N; hAPN; AP-M

NCBI Description

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]

Uniprot Description

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization.

Research Articles on hCD13

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Product Notes

The hCD13 anpep (Catalog #AAA668994) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to hCD13 (Clone: BF10) FITC Conjugated reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's hCD13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: 0.2-0.5 ug/10^6 cells. Researchers should empirically determine the suitability of the hCD13 anpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCD13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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