hCD154 recombinant protein
Recombinant hCD154-muCD8 R-PE Conjugate
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Synonyms
hCD154; Recombinant hCD154-muCD8 R-PE Conjugate; CD154 (CD40L)-muCD8 R-PE Conjugate; CD40L; CD40 Ligand; gp39; hCD154 recombinant protein
Sequence Length
260
Applicable Applications for hCD154 recombinant protein
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
Human CD154-muCD8 binds to cell surface expressed human CD40 and this binding is blocked by anti-human CD154 monoclonal antibody. Recombinant CD154-muCD8 has been shown to induce phosphorylation of ERK, JNK and p38 molecules and subsequent activation of NFkB pathway (14, 15, 16) and to stimulate B cell proliferative response (19, 21). It crossreacts with CD40 in other species including pig (17)and dog(18).
Transfectant Cell Line
CHO
Molecular Structure
A soluble molecule consisting of the extracellular domain (213aa) of human CD154 fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric molecular weight of 42.6 kd. A similar construct described in reference (9).
Conjugate
PE
Related Product Information for hCD154 recombinant protein
Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. It can undergo proteolytic cleavage into a 19kD immunologically active soluble form. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). Increased levels of CD154 has been associated with autoimmune disorders including SLE, CLL and eosinophilic fasciitis (5,9,10,11). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells, macrophages and activated platelets indicating a role for the CD40-CD154 immunoregulatory signaling in arthrosclerosis and cardiovascular disorders (7,12,13).
Product Categories/Family for hCD154 recombinant protein
References
1)D. Gray, et al, (1994) Seminars in Immunol 6: 303-310. 2) A.C. Grammer, et al, (1995) J Immunol 154: 4996-5010. 3) F. Pietravalle, et al, (1996) J Biol Chemistry 271: 5965-5967. 4) R.J. Noelle, (1996) Immunity 4: 415-419. 5) A. Desai-Mehta, et al, (1996) J Clin Invest 97: 2063-2073. 6) I.S. Grewal and R.A. Flavell, (1996) Immunol Today 17: 410-414. 7) F. Mach, et al, (1997) Proc Natl Acad Sci USA 94:1931-1936. 8) A.C. Grammer, et al, (1999) J Immunol 163: 4150-4159. 9) D. Hollenbaugh, (1992) EMBO 11: 4314-4321. 10) R.K. Vakkalanka, et al, (1999) Arthritis Rhem 42:871-81. 11) M. Jinnin, et al.(2003) Ann Rhem Dis 62: 190-191. 12) U. Schonbeck, et al, (2000) PNAS USA 97: 7458-7463. 13) U. Schonbeck, et al, (2001) Circulation 104: 2266-2268. 14) AC Grammer, PF Lipsky, et al (2000) Advances in Immunology 76: 61-178. 15) AC Grammer, PF Lipsky, et al (2004) Arthristis Research and Therapy 6: 28-38. 16). Melter M, D M Briscoe, et al (2000) Blood 96:3801-3808. 17). Takamatsu H, RME Parkhouse, et al. (1999) Immunology 97(2):211-213. 18) Daisuke I, J F Modiano, et al. (2012) Leukemia and Lymphoma 53(7): 1390-1398 19) Brodeur SR, RS Geha, et al. (2003) Immunity 18(6): 837-848 20) KC Bankert, GA Bishop, et al.(2015) J Immunol 194(9): 4319-4327. PMID: 25795759.ID: 21) CR Smulski, S Fournel, et al. (2017) Cell Death and Disease 8(2):e2601. doi: 10.1038/cddis.2017.22. PMID: 28182009
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
cd40lg
NCBI Protein Information
CD40 ligand
Research Articles on hCD154
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Product Notes
The hCD154 (Catalog #AAA666938) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's hCD154 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). Human CD154-muCD8 binds to cell surface expressed human CD40 and this binding is blocked by anti-human CD154 monoclonal antibody. Recombinant CD154-muCD8 has been shown to induce phosphorylation of ERK, JNK and p38 molecules and subsequent activation of NFkB pathway (14, 15, 16) and to stimulate B cell proliferative response (19, 21). It crossreacts with CD40 in other species including pig (17)and dog(18). Researchers should empirically determine the suitability of the hCD154 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCD154, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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