Mouse anti-Human CD6 Monoclonal Antibody | anti-CD6 antibody

anti-human CD6-Purified (with Antibiotics)

Cat. Num. AAA666676

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified (with Antibiotics)
• Synonyms: CD6; Monoclonal Antibody; anti-human CD6-Purified (with Antibiotics); Monoclonal anti-human CD6; anti-CD6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 3F7B6
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 143

• Applicable Applications for anti-CD6 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), FX
• Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD6 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 82% sub population of the cells stained positive with a mean shift of 1.34 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD6
• Immunogen: Human rheumatoid synovial T cell line (ST-1)
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD6 antibody

Information: Human CD6 is expressed on peripheral blood T cells, most thymocytes and a subset of B cells. CD6 is similar to CD5 and contains three cysteine-rich domains with structural homology to the macrophage scavenger receptor. CD6 is involved in T cell activation and its ligand was recently identified. Antibody UMCD6 recognizes CD6 molecules of approximately 130 kd. Antibody UMCD6 is a useful reagent to study T cell activation through CD6.

References

C.M. Bott, et al, (1993) Int Immunol 5: 783-792. M.A. Bowen, et al, (1995) J Exp Med 181: 2213-2220. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 419-422. G.S. Whitney, et al, (1995) J Biol Chem 270: 18187-18190.

NCBI and Uniprot Product Information

• NCBI GI #: 13111306
• NCBI GeneID: 282881
• Molecular Weight: 15,814 Da
• NCBI Official Full Name: CD6, partial
• NCBI Official Synonym Full Names: CD6 molecule<
• NCBI Official Symbol: CD6
• NCBI Protein Information: T-cell differentiation antigen CD6; CD6 molecule; CD6 antigen
• UniProt Protein Name: CD6
• Protein Family: CD63 antigen
• UniProt Entry Name: Q9BH12_BOVIN