Mouse CD154 Monoclonal Antibody | anti-CD154 antibody

anti-human CD154-Purified Preservative Free

Cat. Num. AAA666553

• Reactivity: Human, Primate
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
• Purity: Purified Preservative Free
• Synonyms: CD154; Monoclonal Antibody; anti-human CD154-Purified Preservative Free; Monoclonal anti-human CD154 (CD40 Ligand); anti-CD154 antibody

• Host: Mouse
• Reactivity: Human, Primate
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 24-31
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for anti-CD154 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistchemistry (IHC)
• Performance: Five x 105 Jurkat-4G (a CD154+ subclone of Jurkat) cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD154 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 sterile filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
• Immunogen: Human sgp39 fusion protein
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended. Open under aseptic conditions.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD154 antibody

Information: Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. It can undergo proteolytic cleavage into an immunologically active soluble form. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). Increased levels of CD154 has been associated with autoimmune disorders including SLE, CLL and eosinophilic fasciitis (5,9,10,11). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells, macrophages and activated platelets indicating a role for the CD40-CD154 immunoregulatory signaling in artherosclerosis and cardiovascular disorders (7,12,13). Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd. The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation.

References

1) D. Gray, et al, (1994) Seminars in Immunol 6: 303-310. 2) A.C. Grammer, et al, (1995) J Immunol 154: 4996-5010. 3) F. Pietravalle, et al, (1996) J Biol Chemistry 271: 5965-5967. 4) R.J. Noelle, (1996) Immunity 4: 415-419. 5) A. Desai-Mehta, et al, (1996) J Clin Invest 97: 2063-2073. 6) I.S. Grewal and R.A. Flavell, (1996) Immunol Today 17: 410-414. 7) F. Mach, et al, (1997) Proc Natl Acad Sci USA 94:1931-1936. 8) A.C. Grammer, et al, (1999) J Immunol 163: 4150-4159. 9) D. Hollenbaugh, (1992) EMBO 11: 4314-4321. 10) R.K. Vakkalanka, et al, (1999) Arthritis Rhem 42:871-81. 11) M. Jinnin, et al.(2003) Ann Rhem Dis 62: 190-191. 12) U. Schonbeck, et al, (2000) PNAS USA 97: 7458-7463. 13) U. Schonbeck, et al, (2001) Circulation 104: 2266-2268.