Mouse anti-Human CD53 Monoclonal Antibody | anti-CD53 antibody

anti-human CD53-Purified (with Antibiotics)

Cat. Num. AAA666456

• Gene Names: CD53; MOX44; TSPAN25
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified (with Antibiotics)
• Synonyms: CD53; Monoclonal Antibody; anti-human CD53-Purified (with Antibiotics); Monoclonal anti-human CD53; anti-CD53 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 63.5A3
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 219

• Applicable Applications for anti-CD53 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured human Jurkat cells per tube were incubated 45 minutes on ice with 80 ul of anti-CD53 antibody at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human CD53
• Immunogen: Human sezary cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD53 antibody

Information: Human CD53 is a tetraspan cell surface glycoprotein found on all hemopoetic cells except platelets and red blood cells. Antibody 63.5A3 recognizes the CD53 molecule of about 32-42 kd.

References

1.) Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 534, 541. 2.) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 556-559. 3.) Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 517-519.

NCBI and Uniprot Product Information

• NCBI GI #: 26251884
• NCBI GeneID: 963
• Molecular Weight: 24,341 Da
• NCBI Official Full Name: CD53 molecule
• NCBI Official Synonym Full Names: CD53 molecule
• NCBI Official Symbol: CD53
• NCBI Official Synonym Symbols: MOX44; TSPAN25
• NCBI Protein Information: leukocyte surface antigen CD53; tspan-25; CD53 antigen; tetraspanin-25; CD53 glycoprotein; cell surface antigen; CD53 tetraspan antigen; transmembrane glycoprotein; cell surface glycoprotein CD53; antigen MOX44 identified by monoclonal antibody MRC-OX44
• UniProt Protein Name: Leukocyte surface antigen CD53
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD53
• UniProt Synonym Gene Names: MOX44; TSPAN25; Tspan-25
• UniProt Entry Name: CD53_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CD53: May be involved in growth regulation in hematopoietic cells. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; integral to membrane; plasma membrane; immunological synapse; intercellular junction

Molecular Function: protein binding

Biological Process: signal transduction