ASK1, aa1276-1291 Peptide | ASK1 peptide

ASK1, aa1276-1291, Human (Apoptosis Signal-regulating Kinase 1, ASK-1, Mitogen-activated Protein Kinase Kinase Kinase 5, MAP3K5, MAPKKK5, MAPK/ERK Kinase Kinase 5, MEK Kinase 5, MEKK 5, MEKK5)

Cat. Num. AAA659107

• Applications: Western Blot
• Purity: Highly Purified; > 90% by HPLC
• Synonyms: ASK1; aa1276-1291; Human (Apoptosis Signal-regulating Kinase 1; ASK-1; Mitogen-activated Protein Kinase Kinase Kinase 5; MAP3K5; MAPKKK5; MAPK/ERK Kinase Kinase 5; MEK Kinase 5; MEKK 5; MEKK5); ASK1 peptide

• Host: Synthetic peptide
• Purity/Purification: Highly Purified; > 90% by HPLC
• Form/Format: Supplied as a lyophilized powder.

• Applicable Applications for ASK1 peptide: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Stable for 12 months at -20 degree C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ASK1 peptide

Synthetic peptide (EEKDQEIKHLKLKSQP-NH2) (linear) corresponding to aa1276-1291 of human ASK1. The peptide has an amide group blocking the carboxyl terminus.

Product Categories/Family for ASK1 peptide

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 269970311
• NCBI GeneID: 853814
• NCBI Accession #: NP_012872.2
• NCBI GenBank Nucleotide #: NM_001179618.1
• UniProt Accession #: P35734; Q68EC3; Q6B290; D6VXN5
• Molecular Weight: 32,072 Da
• NCBI Official Full Name: Ask1p
• NCBI Official Symbol: ASK1
• NCBI Protein Information: Ask1p
• UniProt Protein Name: DASH complex subunit ASK1
• Protein Family: DASH complex
• UniProt Gene Name: ASK1
• UniProt Entry Name: ASK1_YEAST

Uniprot Description

Function: Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. Ref.11 Ref.12 Ref.14

Subunit structure: The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1. Ref.13

Subcellular location: Nucleus. Cytoplasm › cytoskeleton › spindle. Chromosome › centromere › kinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.9

Miscellaneous: Present with 2836 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the DASH complex ASK1 family.