NFkB, p65 Peptide | NKIRAS2 peptide

NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA), Control Peptide

Cat. Num. AAA658607

• Gene Names: NKIRAS2; KBRAS2; kappaB-Ras2
• Purity: Purified; Purified by HPLC
• Synonyms: NFkB; p65; p65 (NF-kB; Nuclear Factor kappa B; RelA); Control Peptide; NKIRAS2 peptide

• Purity/Purification: Purified; Purified by HPLC
• Form/Format: Supplied as a liquid, 0.01% sodium azide.

• Application Notes: Suitable for use to block specific interaction of antiNFkB p65 with the NFkB p65 subunit.; Dilution: Blocking: Interaction: 1ug per 1ul of antiserum used.; Optimal working dilutions to be determined by researcher.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. If the product contains a precipitate, microcentrifuge before use.

Related Product Information for NKIRAS2 peptide

The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so-called ankyrin repeat at their C-termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Product Categories/Family for NKIRAS2 peptide

Molecular Biology; MB-Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 222136632
• NCBI GeneID: 28511
• NCBI Accession #: NP_001138401.1
• NCBI GenBank Nucleotide #: NM_001144929.1
• UniProt Accession #: Q9NYR9; Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 2
• NCBI Official Symbol: NKIRAS2
• NCBI Official Synonym Symbols: KBRAS2; kappaB-Ras2
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 2
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS2
• UniProt Synonym Gene Names: KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
• UniProt Entry Name: KBRS2_HUMAN

Uniprot Description

Function: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB

By similarity.

Subunit structure: Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Ref.1

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Widely expressed. Ref.1

Domain: In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.

Sequence similarities: Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

Sequence caution: The sequence BAB55006.1 differs from that shown. Reason: Absence of residues from position 133 within an exon that change the frame which is not the result of an alternative splicing.