Testing Data
(Example: The Mutiple Tag was loaded on a SDS gel and the 40 kDa fusion protein was detected using rabbit anti VSV-g antibody.)
Multiple Tag Cell Lysate
Multiple Tag Cell Lysate
Applications
Western Blot, Gel Super Shift Assay
Purity
LysatePurified lysate
Synonyms
Multiple Tag Cell Lysate; Multiple Tag Cell Lysate lysate
Purity/Purification
Lysate
Purified lysate
Purified lysate
Form/Format
Supplied as a lyophilized powder. Reconstitute with 200ul of ddH2O.
Applicable Applications for Multiple Tag Cell Lysate lysate
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western Blot and Dot Blot.
Dilution: Western Blot: 0.04-0.2ul per lane of minigel. Dilute with PBS.
Dilution: Western Blot: 0.04-0.2ul per lane of minigel. Dilute with PBS.
Preparation and Storage
Reconstitute to nominal volume by adding sterile dH2O. Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Reconstituted product is stable for 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Example: The Mutiple Tag was loaded on a SDS gel and the 40 kDa fusion protein was detected using rabbit anti VSV-g antibody.)
Testing Data
(Example: The Mutiple Tag was loaded on a SDS gel and the 40 kDa fusion protein was detected using rabbit anti VSV-g antibody.)
Related Product Information for Multiple Tag Cell Lysate lysate
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. This fusion protein is of 52kD in mass. It contains 16 commonly used tags, which are listed on the table below, from the N-terminus to the C-terminus. Tag Sequence Trx
T7 CMASMTGGQQMG; HSV QPELAPEDPEDC; c-Myc EQKLISEEDLC; VSV-g YTDIEMNRLGK; Glu-Glu CEEEEYMPME; V5 GKPIPNPLLGLDST; E-tag GAPVPYPDPLEPR; DYKDDDDK; S-tag KETAAAKFERQHMDS; HA YPYDVPDYAC; KT3 KPPTPPPEPET; E2 GVSSTSSDFRDR; AU1 DTYRYI; AU5 TDFYLK; 6x His HHHHHH
T7 CMASMTGGQQMG; HSV QPELAPEDPEDC; c-Myc EQKLISEEDLC; VSV-g YTDIEMNRLGK; Glu-Glu CEEEEYMPME; V5 GKPIPNPLLGLDST; E-tag GAPVPYPDPLEPR; DYKDDDDK; S-tag KETAAAKFERQHMDS; HA YPYDVPDYAC; KT3 KPPTPPPEPET; E2 GVSSTSSDFRDR; AU1 DTYRYI; AU5 TDFYLK; 6x His HHHHHH
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Product Notes
The Multiple Tag Cell Lysate (Catalog #AAA657466) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Multiple Tag Cell Lysate can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot and Dot Blot. Dilution: Western Blot: 0.04-0.2ul per lane of minigel. Dilute with PBS. Researchers should empirically determine the suitability of the Multiple Tag Cell Lysate for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Multiple Tag Cell Lysate, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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