Apolipoprotein A-I Native Protein
Apolipoprotein A-I (Apo A-I)
Purity
>99% (SDS-PAGE). Isolated by ultracentrifugal flotation between densities 1.063–1.21g/ml, lyophilized, then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride and ~25mM
Synonyms
Apolipoprotein A-I; Apolipoprotein A-I (Apo A-I); Apolipoprotein A-I native protein
Host
High-density lipoprotein (HDL) from pooled human plasma
Purity/Purification
>99% (SDS-PAGE). Isolated by ultracentrifugal flotation between densities 1.063–1.21g/ml, lyophilized, then subjected to repeated ethanol extraction. The precipitated protein was dried under nitrogen then dissolved in 6M guanidine hydrochloride and ~25mM
Form/Format
Supplied as a liquid containing 3M guanidine hydrochloride, 10mM Tris, 100mM sodium chloride, 1mM EDTA and 1mM sodium azide, pH 7.4.
Concentration
~1mg/ml (varies by lot)
Preparation of Protein
Apo AI can be renatured into PBS, TBS, or other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14,000 or less. This should be performed in a cold room.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge The original vial after thawing and prior to removing the cap. Note: Dialyzed Apo AI in PBS or TBS with 1mM EDTA and 0.1% sodium azide may be stored for up to 2 weeks at 4 degree C. Storage of Apo AI in PBS or TBS at -20 degree C may lead to precipitation when thawed.
Product Categories/Family for Apolipoprotein A-I native protein
References
1.Chen, W., et al., (2001), The Journal of Biological Chemistry, 276(47): 43564-43569 2.Feng, B., et al., (2002), The Journal of Biological Chemistry, 277(45): 43271-43280 3.Gerbod-Giannone, M.C., et al., (2006), PNAS, 103(9): 3112-3117 4.Martinez, L.O., (2003), The Journal of Biological Chemistry, 278(39): 37368-37374 5.Sun, Y., et al., (2003), The Journal of Biological Chemistry, 278(8): 5813-5820 6. Ishiguro, H., et al., (2001), The Journal of Biological Chemistry, 276(39): 36742-36748.
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Product Notes
The Apolipoprotein A-I (Catalog #AAA656941) is a Native Protein produced from High-density lipoprotein (HDL) from pooled human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Apolipoprotein A-I, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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