Bacillus anthracis Lethal Factor Protease Substrate 2 Infectious Disease
Bacillus anthracis Lethal Factor Protease Substrate 2 (LFPS2) (Anthrax)
Purity
Highly Purified97% by HPLC
Synonyms
Bacillus anthracis Lethal Factor Protease Substrate 2; Bacillus anthracis Lethal Factor Protease Substrate 2 (LFPS2) (Anthrax); Bacillus anthracis Lethal Factor Protease Substrate 2 infectious disease
Purity/Purification
Highly Purified
97% by HPLC
97% by HPLC
Immunogen
Lethal factor Protease Substrate 2, pNA derivative MEk2 peptide substrate for high-throughput screening of LF inhibitors (14aa)
Sequence (linear)
Ac-Gly-Tyr-bAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-pNA
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Stable for 12 months at -20 degree C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Bacillus anthracis Lethal Factor Protease Substrate 2 infectious disease
Lethal factor Protease Substrate 2, pNA derivative MEk2 peptide substrate for high-throughput screening of LF inhibitors (14aa) After inhalation by mammals, Bacillus anthracis spores germinate in alveolar macrophages then migrate to lymph nodes where they multiply. The vegetative bacteria excrete the tripartite exotoxin which consists of three polypeptides: protective antigen (PA, 83kD), lethal factor (LF, 90kD) and oedma factor (OF, 89kD). The two components (OF and LF) of the toxin enzymatically modify substrates within the cytosol of the mammalian cells: The OF is an adenylate cyclase that impairs the host defenses through a variety of mechanisms inhibiting phagocytosis. The LF is a zinc dependent protease that cleaves several mitogen activated protein kinase kinases (MAPKK) and causes lysis of macrophages. To intoxicate mammalian cells, the third component of the toxin PA, binds to a ubiquitously expressed cellular receptor, Tumor Endothelium Marker-8 (TEM8). Upon binding to TEM8, PA is cleaved into 20 and 63kDa fragments (PA20 and PA63) by furin or furin-like proteases. PA20 dissociates into medium and allows the PA63 fragment to heptamerize and bind LF and OF of the toxin. The resulting complex of PA63 fragment with EF and/or OF binds to PA-receptor TEM8/ATR and internalized into endosomes followed by translocation of LF and OF into cytosol of the cells.
Anthrax lethal toxin produced by the bacterium Bacillus anthracis is the major cause of death in animals infected with anthrax.One component of this toxin, lethal factor (LF), inactivates membersof the mitogen-activated protein kinase kinase or MEK family throughproteolysis of their NH2 termini. Although LF has been shown to cleave the NH2 termini of select members of the mitogen-activated protein kinase kinase or MEKfamily, the substrate requirements that determine LF specificityare unknown. Indirect evidence suggests that epitopes distal to the cleavage site are required for LF-MEK interaction.
Anthrax lethal toxin produced by the bacterium Bacillus anthracis is the major cause of death in animals infected with anthrax.One component of this toxin, lethal factor (LF), inactivates membersof the mitogen-activated protein kinase kinase or MEK family throughproteolysis of their NH2 termini. Although LF has been shown to cleave the NH2 termini of select members of the mitogen-activated protein kinase kinase or MEKfamily, the substrate requirements that determine LF specificityare unknown. Indirect evidence suggests that epitopes distal to the cleavage site are required for LF-MEK interaction.
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Product Notes
The Bacillus anthracis Lethal Factor Protease Substrate 2 (Catalog #AAA655151) is an Infectious Disease and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Bacillus anthracis Lethal Factor Protease Substrate 2, Infectious Disease" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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