Amylase, alpha Pancreas Enzyme | AMY2A enzyme

Amylase, alpha, Human Pancreas

Cat. Num. AAA653881

• Gene Names: AMY2A; PA; AMY2; AMY2B
• Purity: Purified; Purified
• Synonyms: Amylase; alpha Pancreas; alpha; Human Pancreas; AMY2A enzyme

• Host: Human Pancreas
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 100mM Tris-HCl, 150mM sodium chloride, 1mM calcium chloride, 1mM benzamidine, 1mM pefabloc, pH 7.5. No preservatives added.

• CAS Number: 9000-90-2
• Activity: 240 units/ml @ 37 degree C; ; Specific Activity:; 240u/mg
• Contaminants: GOT: 0.002%; ALP: 0.04%; Lipase: <0.001%; Protease:< 0.001%
• Unit Definition: One unit will catalyze the hydrolysis of 1umole of 2-chloro-4-nitrophenyl-a-D-maltotrise per minute at 37ºC
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Related Product Information for AMY2A enzyme

Alpha amylase is able to catalyze the hydrolysis of starch (amylose and amylopectin) to a significant extent resulting in the release of maltose. The enzyme is found in nearly all plants, animals and microorganisms. The enzymes from various sources exhibit marked differences in physical, chemical and catalytic properties. Bacterial amylases and those from porcine pancreas and human saliva have been extensively studied. Alpha amylase is widely used in the food industry as a clarifying agent. Determination of serum alpha amylase activity, for the identification of acute pancreatitis, is a widely used procedure in clinical medicine (Brooks, F., New Eng. J. Med., 286, 300, 1972). Additionally, it has been reported that elevated alpha amylase activity in serum also occurs in mumps, renal disease and abdominal disorders such as cholecystitis (Salt, W. and Schenkder, S., Medicine, 55, 269, 1978).

Product Categories/Family for AMY2A enzyme

Molecular Biology; MB-Enzymes; Glycosidase

NCBI and Uniprot Product Information

• NCBI GI #: 529397
• NCBI GeneID: 279
• UniProt Accession #: P04746; Q9UBH3
• Molecular Weight: 57,707 Da
• NCBI Official Full Name: amylase
• NCBI Official Synonym Full Names: amylase, alpha 2A (pancreatic)
• NCBI Official Symbol: AMY2A
• NCBI Official Synonym Symbols: PA; AMY2; AMY2B
• NCBI Protein Information: pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
• UniProt Protein Name: Pancreatic alpha-amylase
• UniProt Gene Name: AMY2A
• UniProt Synonym Gene Names: PA
• UniProt Entry Name: AMYP_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor: Binds 1 calcium ion per subunit.Binds 1 chloride ion per subunit.

Subunit structure: Monomer.

Subcellular location: Secreted › extracellular space.

Sequence similarities: Belongs to the glycosyl hydrolase 13 family.