Exonuclease I, E. coli Enzyme | T7p35 enzyme
Exonuclease I, E. coli (ExoI)
Purity
Molecular Biology Grade
Synonyms
Exonuclease I; E. coli; E. coli (ExoI); T7p35 enzyme
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1mM EDTA, 1mM DTT and 50% glycerol.
Application Notes
Suitable for use in the purification of PCR products, removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures and assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus.
Supplied With
E9007-85C1: 10X Reaction Buffer: Supplied as a liquid in 670mM glycine-KOH, pH 9.5 at 25 degree C, 67mM MgCl2, 10mM DTT.
Unit Definition
One unit of E9007-85C catalyzes the release of 10 nano-moles of acid soluble nucleotides in 30 min at 37ºC.
Preparation and Storage
-20 degree C
Related Product Information for T7p35 enzyme
The Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact. It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).
Product Categories/Family for T7p35 enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54.5kD
NCBI Official Full Name
exonuclease
NCBI Official Symbol
T7p35
NCBI Protein Information
exonuclease
UniProt Protein Name
Exodeoxyribonuclease
UniProt Gene Name
6
UniProt Synonym Gene Names
Exonuclease
UniProt Entry Name
EXRN_BPT7
Uniprot Description
Function: This enzyme is essential for phage DNA replication; it is believed to function in the removal of DNA-linked RNA primers. It is also necessary for host DNA degradation and phage genetic recombination.
Catalytic activity: Exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 5'-phosphates.
Similar Products
Product Notes
The T7p35 6 (Catalog #AAA653833) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in the purification of PCR products, removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures and assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus. Researchers should empirically determine the suitability of the T7p35 6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Exonuclease I, E. coli, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
