Tyrosine Decarboxylase, Holoenzyme
Tyrosine Decarboxylase, Holoenzyme
Purity
Molecular Biology GradeQuality Control: SDS-PAGE
Synonyms
Tyrosine Decarboxylase; Holoenzyme; Holoenzyme enzyme
Host
Streptococcus faecalis (NCTC6783)
Specificity
Dihydroxyphenylalanine and L-phenylalanine are acted upon at a much slower rate than L-tyrosine.
Purity/Purification
Molecular Biology Grade
Quality Control: SDS-PAGE
Quality Control: SDS-PAGE
Form/Format
Supplied as lyophilized cells.
Activity
0.1units/mg dw
Unit Definition
1 unit yields 1umole of CO2 per minute from L-tyrosine @ 37 degree C, pH 5.5.
Preparation and Storage
-20 degree C
Related Product Information for Tyrosine Decarboxylase, Holoenzyme enzyme
Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary coenzyme. By utilizing the apoenzyme (TYDAPO), prepared from cells grown on a vitamin B6 deficient medium, pyridoxal phosphate may be determined. Decarboxylation of radio-labeled tyrosine has permitted the determination of much smaller amounts of pyridoxal phosphate.
Acetone-dried cells were used as the source in a relatively simple extraction and purification of the apoenzyme. The holoenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically. Tashian (1957) has reported a determination of tyrosine in urine based on this principle. Plasma phenylalanine can also be determined using this enzyme. Poisner (1960) determined serum phenylalanine levels in schizophrenics using Hsia's method and obtained a significant elevation over normals.
Acetone-dried cells were used as the source in a relatively simple extraction and purification of the apoenzyme. The holoenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically. Tashian (1957) has reported a determination of tyrosine in urine based on this principle. Plasma phenylalanine can also be determined using this enzyme. Poisner (1960) determined serum phenylalanine levels in schizophrenics using Hsia's method and obtained a significant elevation over normals.
Product Categories/Family for Tyrosine Decarboxylase, Holoenzyme enzyme
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Product Notes
The Tyrosine Decarboxylase, Holoenzyme (Catalog #AAA653815) is an Enzyme produced from Streptococcus faecalis (NCTC6783) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tyrosine Decarboxylase, Holoenzyme, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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