Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol Enzyme
Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)
Purity
Molecular Biology Grade
Synonyms
Zymolyase 20T Concentrate 50mg/ml; 0.1M Sorbitol; 0.1M Sorbitol (Lyticase; Yeast Lytic Enzyme); 0.1M Sorbitol enzyme
Purity/Purification
Molecular Biology Grade
Form/Format
Pale yellow to brown solution/suspension
Activity: 20u/mg
Purity: Purified by ammonium sulfate precipitation.
Unit Definition:
One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800).
Activity: 20u/mg
Purity: Purified by ammonium sulfate precipitation.
Unit Definition:
One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800).
Application Notes
Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation
CAS Number
37340-57-1
Water
Molecular Biology Grade, RNase, DNase-Free
Filter: 0.2um validated
Sorbitol: 98%
Filter: 0.2um validated
Sorbitol: 98%
Note
Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension.
Preparation and Storage
Stable for 12 months at -20 degree C.
Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Testing Data
(The extracellular domain is required for Sln1 kinase activation by wall perturbations. A, representative field showing localization of SLN1-GFP (pSS1881) or sln1ΔECD-GFP (pSS1904). BF, bright field. B, Western analysis showing reduced levels of sln1ΔECD compared with full-length SLN1. SLN1, JF2007 (sln1Δ) carrying full-length SLN1-myc (pCLM994); sln1ΔECD, JF2007 carrying sln1ΔECD-myc (pCLM1774). C, comparison of Hog1 phosphorylation kinetics in SLN1 (JF2008 (sln1Δ sho1Δ) carrying full-length (pCLM994)) and sln1ΔECD (JF2008 (sln1Δ sho1Δ) carrying sln1ΔECD (pCLM1774)) strains following addition of 0.4 M NaCl. D, Northern (RNA) hybridization analysis of RNA prepared from wild type cultures from the sln1Δ strain, JF2007, or sln1Δ ccw12Δ strain, JF2368, transformed with the SLN1 expression plasmids, pCLM994 (SLN1) or pCLM1774 (sln1ΔECD), and treated with 1 unit/ml zymolyase (+) where indicated. NCA3 expression was normalized to expression of the SLN1-SKN7-independent CDC33 gene. The extent of induction by zymolyase is shown relative to the untreated SLN1 or sln1ΔECD strains.)
Related Product Information for Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol enzyme
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is * Lyticase is similar but testing has shown that Zymolyase exhibits greater lytic activity.
Product Categories/Family for Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol enzyme
Similar Products
Product Notes
The Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Catalog #AAA653686) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. Protoplast/Spheroplast Preparation, Yeast Cell Fusion, Yeast Cell Transformation. Researchers should empirically determine the suitability of the Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
