Amylase, alpha Saliva Enzyme | AMY1A enzyme

Amylase, alpha, Human Saliva

Cat. Num. AAA653629

• Gene Names: AMY1A; AMY1
• Purity: Purified; 90% pure (SDS-PAGE).
• Synonyms: Amylase; alpha Saliva; alpha; Human Saliva; AMY1A enzyme

• Host: Amylase alpha, from human saliva.
• Purity/Purification: Purified; 90% pure (SDS-PAGE).
• Form/Format: Supplied as a lyophilized powder from 150mM sodium chloride, 20mM Tris, 1mM calcium chloride, pH 7.2. Reconstitute with Tris saline or other buffer.

• Activity: ~120 Units/mg powder @ 37 degree C; ; Protein:; ~0.4mg Protein/mg powder (Coomassie); ; Specific Activity:; ~300 Units/mg Protein; ; Unit Definition:; One unit will catalyze the hydrolysis of one micromole of malto pentaose, which through coupled reactions results in the formation of 5 micromoles of glucose-6-phosphate per minute at 37 degree C. Measured at 340nm as an equimolar amount of NADH produced by a coupled reaction.
• Contaminants: Lipase: <0.001%; Proteases: <0.001%; Ammonia: <0.001Umole/mg
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for AMY1A enzyme

Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary starch and glycogen. Alternative splicing results in multiple transcript variants encoding the same protein.

Product Categories/Family for AMY1A enzyme

Molecular Biology; MB-Enzymes; Glycosidase

NCBI and Uniprot Product Information

• NCBI GI #: 1351933
• NCBI GeneID: 276
• UniProt Accession #: P04745; Q13763; Q5T083; A6NJS5; A8K8H6
• Molecular Weight: 57,768 Da
• NCBI Official Full Name: Alpha-amylase 1
• NCBI Official Synonym Full Names: amylase, alpha 1A (salivary)
• NCBI Official Symbol: AMY1A
• NCBI Official Synonym Symbols: AMY1
• NCBI Protein Information: alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A; amylase, salivary, alpha-1A; 1,4-alpha-D-glucan glucanohydrolase 1
• UniProt Protein Name: Alpha-amylase 1
• UniProt Gene Name: AMY1A
• UniProt Synonym Gene Names: AMY1
• UniProt Entry Name: AMY1_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor: Binds 1 calcium ion per subunit.Binds 1 chloride ion per subunit.

Subunit structure: Monomer.

Subcellular location: Secreted.

Sequence similarities: Belongs to the glycosyl hydrolase 13 family.