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Keratinocyte Basal Medium MCDB 153 Culture Media

Keratinocyte Basal Medium MCDB 153 (Powder)

Purity
Cell Culture Grade
Synonyms
Keratinocyte Basal Medium MCDB 153; Keratinocyte Basal Medium MCDB 153 (Powder); Keratinocyte Basal Medium MCDB 153 culture media
Ordering
For Research Use Only!
Purity/Purification
Cell Culture Grade
Endotoxin Level
1EU/ml
pH
5.8 ± 0.4
Preparation and Storage
RT/4 degree C
Related Product Information for Keratinocyte Basal Medium MCDB 153 culture media
MCDB media were designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium was formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promoted growth of a specific cell type. MCDB 105 and 110 are modifications of MCDB 104 medium, optimized for long-term survival and rapid clonal growth of human diploid fibroblast-like cells (WI-38, MRC-5, IMR-90) and of low-passage human foreskin fibroblasts using FBSP or hormone and growth factor supplements. MCDB 151, 153, 201 and 302 are modifications of Ham's nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts and chinese hamster ovary (CHO) cells using low levels of FBSP, extensive trace elements or no serum protein. The selection of a nutrient medium is strongly influenced by 1. type of cell; 2. type of culture (monolayer, suspension, clonal); and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line. MCDB 153 is a basal medium for the growth of normal keratinocytes and squamous epithelial cells after growth factor supplementation. Calcium content is low. Supplement with growth factors or fetal calf serum.
Product Categories/Family for Keratinocyte Basal Medium MCDB 153 culture media

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Product Notes

The Keratinocyte Basal Medium MCDB 153 (Catalog #AAA653431) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Keratinocyte Basal Medium MCDB 153, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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