Fischer's Medium Culture Media
Fischer's Medium (Leukemic Cells of Mice) w/L-Glutamine (Powder)
Purity
Cell Culture Grade
Synonyms
Fischer's Medium; Fischer's Medium (Leukemic Cells of Mice) w/L-Glutamine (Powder); Fischer's Medium culture media
Purity/Purification
Cell Culture Grade
Form/Format
White to pale orange, homogeneous, free flowing powder
Endotoxin Level
1EU/ml
Solubility
Orange, clear, complete.
Directions per Liter
Dissolve 10.6g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add 1.125g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
pH
6.87
Directions per Liter
Dissolve 1 0.6g in SOO-900ml of ddH20 stirring gently until completely solubilized. If required, add 1.125g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution I to 1 L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Inorganic Salts:
Calcium Chloride'2H20 = 91.000
Magnesium Chloride'6H20 = 100.000
Potassium Chloride = 400.000
Sodium Chloride = 8000.000
Sodium Phosphate Dibasic = 60.000
Sodium Phosphate Monobasic = 60.000
Amino Acids:
L-Arginine'HCI = 15.000
L –Asparagine =10.000
L-Cystine'2HCI = 26.100
L-Glutamine = 204.000
L-Histidine'HCI'H20 = 81.000
L-Isoleucine =75.000
L-Leucine = 30.000
L-Lysine'HCI = 50.000
L-Methionine =100.000
L-Phenylalanine = 60.000
L-Serine = 15.000
L-Threonine = 40.000
L-Tryptophan = 10.000
L-Tyrosine'2Na'2H20 = 87.160
L-Valine = 70.000
Vitamins:
D-Biotin = 0.010
Choline Chloride = 1.500
Folic Acid = 10.000
Myo-Inositol = 1.500
Niacinamide = 0.500
D-Pantothenic Acid'Ca = 0.500
Pyridoxal'HCI = 0.500
Riboflavin = 0.500
Thiamine-HCl = 1.000
Other:
D-Glucose = 1000.000
Phenol Red, Sodium = 5.300
Total: 10.606g/L
Inorganic Salts:
Calcium Chloride'2H20 = 91.000
Magnesium Chloride'6H20 = 100.000
Potassium Chloride = 400.000
Sodium Chloride = 8000.000
Sodium Phosphate Dibasic = 60.000
Sodium Phosphate Monobasic = 60.000
Amino Acids:
L-Arginine'HCI = 15.000
L –Asparagine =10.000
L-Cystine'2HCI = 26.100
L-Glutamine = 204.000
L-Histidine'HCI'H20 = 81.000
L-Isoleucine =75.000
L-Leucine = 30.000
L-Lysine'HCI = 50.000
L-Methionine =100.000
L-Phenylalanine = 60.000
L-Serine = 15.000
L-Threonine = 40.000
L-Tryptophan = 10.000
L-Tyrosine'2Na'2H20 = 87.160
L-Valine = 70.000
Vitamins:
D-Biotin = 0.010
Choline Chloride = 1.500
Folic Acid = 10.000
Myo-Inositol = 1.500
Niacinamide = 0.500
D-Pantothenic Acid'Ca = 0.500
Pyridoxal'HCI = 0.500
Riboflavin = 0.500
Thiamine-HCl = 1.000
Other:
D-Glucose = 1000.000
Phenol Red, Sodium = 5.300
Total: 10.606g/L
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.
Related Product Information for Fischer's Medium culture media
Fischer's Medium was originally formulated to support serial propagation of cells from leukemic mice. In initial studies, cells in culture were examined for resistance to chemotherapeutic agents. While most of such studies were carried out using whole animals, Fischer's Medium made it possilbe to conduct parallel studies in vitro. Fischer's Medium supports clonal reproduction of cells, particularly lymphoblasts from primary explants or from cells in culture.
Product Categories/Family for Fischer's Medium culture media
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Product Notes
The Fischer's Medium (Catalog #AAA653228) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Fischer's Medium, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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