Edinburgh Minimal Media Phosphate Free Culture Media

Edinburgh Minimal Media Phosphate Free (EMMP) (Powder)

Cat. Num. AAA652754

• Purity: Molecular Biology Grade
• Synonyms: Edinburgh Minimal Media Phosphate Free; Edinburgh Minimal Media Phosphate Free (EMMP) (Powder); Edinburgh Minimal Media Phosphate Free culture media

• Purity/Purification: Molecular Biology Grade

• pH: 6.5
• Solubility: Colorless, clear, complete
• Appearance: White, homogenous, free flowing powder.
• Directions per Liter: Dissolve 29.328 grams per liter of ddH2O. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121ºC (15psi). Note: May also sterilize with 0.2um membrane filter. Aliquot into sterile containers. Contains heat-labile compounds that can be damaged with autoclaving.
• Base: Ammonium Chloride: 5.000; Glucose: 20.000; Sodium Acetate•3H2O: 2.200
• Salt Stock: Calcium Chloride•2H2O: 0.0147; Magnesium Chloride•6H2O: 1.050; Potassium Chloride: 1.000; Sodium Sulfate: 0.040
• Vitamin Stock: Biotin: 0.00001; Inositol: 0.010; Nicotinic Acid: 0.010; Pantothenic Acid•Ca: 0.001
• Mineral Stock: Boric Acid: 0.0005; Citric Acid: 0.001; Copper Sulfate•5H2O: 0.00004; Ferrous Chloride: 0.0002; Manganese Sulfate: 0.0004; Potassium Iodide: 0.0001; Molybdic Acid: 0.00004; Zinc Sulfate•7H2O: 0.0004
• Total: 29.328g/Lit
• Note: The above formulation was derived from the following referenced formula
• Edinburgh Minimal Medium (EMM 2): 3g/L Potassium hydrogen phthalate (14.7mM); 2.2g/L Sodium phosphate dibasic (15.5mM); 5g/L Ammonium chloride (93.5mM); 2% (w/v) Glucose (111mM); 20ml/L Salts (50X stock); 1ml/L Vitamins (1000X stock); 0.1ml/L Minerals (10,000X stock)
• 50X Salts: 52.5g/L Magnesium chloride•6H2O (0.26M); 0.735g/L Calcium chloride•2H2O (4.99mM); 50g/L Potassium chloride (0.67M); 2g/L Sodium sulfate (14.1mM)
• 1000X Vitamins: 1g/L Pantothenic acid (4.20mM); 10g/L Nicotinic acid (81.2mM); 10g/L Inositol (55.5mM); 10mg/L Biotin (40.8uM)
• 10,000X Minerals: 5g/L Boric acid (SO.9mM); 4g/L Manganese sulfate (23.7mM) ); 4g/L Zinc sulfate•7H20 (13.9mM) ); 2g/L Ferrous Chloride (7AOmM) ); 0.4g/L Molybdic acid (2A7mM) ); 1 g/L Potassium iodide (6.02mM) ); 0.4g/L Cupric sulfate.5H20 (1.60mM) ); 10g/L Citric acid (47.6mM)
• Preparation and Storage: Store powdered media ar RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Related Product Information for Edinburgh Minimal Media Phosphate Free culture media

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. Phosphate Free formulation is used for 32P-phosphate labeling.

Sodium phosphate is removed and replaced with potassium hydrogen phthalate with 2g/L sodium acetate (14.6mM). The medium is adjusted to pH 5.5.

Product Categories/Family for Edinburgh Minimal Media Phosphate Free culture media

Culture Media; Media-Yeast