Ribosomal P Antigen
Ribosomal P Antigen
Applications
ELISA
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Ribosomal P Antigen; Ribosomal P Antigen antigen
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 0.01M Tris, 0.53 sodium chloride, 0.02% sodium azide.
Applicable Applications for Ribosomal P Antigen antigen
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Unit Definition
One unit of activity is defined as the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedures for enzyme immunoassay.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Related Product Information for Ribosomal P Antigen antigen
The Ribosomal P antigen consists of three proteins of the 60S ribosomal subunit. These three proteins are designated PO(38kD), P1(19kD), and P2(17kD). Studies have suggested that one P1 homodimer and one P2 homodimer are attached to PO by the NH2-termini. P1 and P2 are believed to be the eukaryotic equivalent of the E. coli ribosomal protein L12 and have been shown to contain sequences that are highly conserved among eukaryotes. The major immunoreactive epitope of these ribosomal antigens has been mapped and is localized to the carboxy terminus. PO, P1, and P2 have an identical 17 amino acid carboxy terminus. The Ribosomal P antigens have GTPase activity and have been shown to be phosphorylated by casein kinase II.
Autoantibodies to Ribosomal antigens were originally described inpatients with Systemic Lupus Erythematosus (SLE), and have been associated with psychotic episodes in SLE. Anti-P antibodies are detected in the sera of 10-20% of SLE patients and are found in 80% of patients with Lupus Psychosis as opposed to SLE patients with other neurological manifestations.
The results of one study have indicated that Anti-P antibodies show moderate restriction in spectrotype, isotype, subclass, and light chain type. It has also been shown that Anti-Ribosomal P antisera may contain antibodies to a small fragment of 28SrRNA. Another study has shown a high frequency of Anti-Ro/SS-A in patients with Anti-P, while Anti-La/SS-B is present only in a low frequency. Other studies have presented evidence that Anti-P antibodies (IgG) may bind to the ribosome in vivo and inhibit protein synthesis. The original method for determining antibodies to ribosomal proteins was immunofluorescence.
Autoantibodies to Ribosomal antigens were originally described inpatients with Systemic Lupus Erythematosus (SLE), and have been associated with psychotic episodes in SLE. Anti-P antibodies are detected in the sera of 10-20% of SLE patients and are found in 80% of patients with Lupus Psychosis as opposed to SLE patients with other neurological manifestations.
The results of one study have indicated that Anti-P antibodies show moderate restriction in spectrotype, isotype, subclass, and light chain type. It has also been shown that Anti-Ribosomal P antisera may contain antibodies to a small fragment of 28SrRNA. Another study has shown a high frequency of Anti-Ro/SS-A in patients with Anti-P, while Anti-La/SS-B is present only in a low frequency. Other studies have presented evidence that Anti-P antibodies (IgG) may bind to the ribosome in vivo and inhibit protein synthesis. The original method for determining antibodies to ribosomal proteins was immunofluorescence.
Product Categories/Family for Ribosomal P Antigen antigen
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Product Notes
The Ribosomal P Antigen (Catalog #AAA651600) is an Antigen and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ribosomal P Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Researchers should empirically determine the suitability of the Ribosomal P Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribosomal P Antigen, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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