Testing Data
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La/SS-B Antigen, Rabbit Thymus Antigen
La/SS-B Antigen, Rabbit Thymus
Applications
ELISA, Western Blot
Purity
Purified by a combination of protein fractionation and column chromatography. Most contaminating antigens have been removed by immunoadsorbent methodology.
Synonyms
La/SS-B Antigen; Rabbit Thymus; Rabbit Thymus antigen
Host
Rabbit thymus
Purity/Purification
Purified by a combination of protein fractionation and column chromatography. Most contaminating antigens have been removed by immunoadsorbent methodology.
Form/Format
Supplied as a lyophilized powder. Reconstitute with 1 mL cold sterile distilled water. Some precipitate may occur but will not affect product performance. Do not store diluted antigen.
Concentration
~10 mg/mL (after reconstitution) (varies by lot)
Applicable Applications for La/SS-B Antigen, Rabbit Thymus antigen
ELISA (EL/EIA), Western Blot (WB)
Activity
Reacts with La/SS-B antisera in immunodiffusion. No reaction is observed with antisera to SM, RNP, SS-A, Scl-70 or Jo-1 using agarose gel double immunodiffusion.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile distilled water. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
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Testing Data
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Related Product Information for La/SS-B Antigen, Rabbit Thymus antigen
The La/SS-B antigen is a 47kD ribonucleic protein that is associated with a spectrum of small RNAs (2). La/SS-B appears to be readily susceptible to proteolysis, resulting in many smaller (42, 320, and 270kD) but still immunoreactive polypeptides (3). It has been shown that the La/SS-B antigen primarily resides in the nucleus (2). It appears to be associated with RNA polymerase III transcripts (4). It has also been shown that the La/SS-B antigen shows strong conservation across species (5). Anti-La/SS-B autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome patients and referred to as SjT.(1) A precipitin with the same properties was rediscovered later and termed La and then SS-B. Anti-La/SS-B precipitins.The presence of autoantibodies against the La/SS-B antigen has been advocated as an aid in Sjogren's Syndrome patients (6). Autoantibodies against La/SS-B are also commonly found in Systemic Lupus Erythematosus and Subacute Cutaneous Lupus (4). In addition, there appears to be a correlation between anti-La/SS-B and the absence of nephritis in SLE patients. Studies have demonstrated that SLE patients with precipitating Anti-Ro/SS-A antibodies have a high incidence of serious nephritis (53%), while those with both Anti-Ro/SS-A and Anti-La/SS-B have a low (9%) frequency of nephritis (7).
Product Categories/Family for La/SS-B Antigen, Rabbit Thymus antigen
Similar Products
Product Notes
The La/SS-B Antigen, Rabbit Thymus (Catalog #AAA651598) is an Antigen produced from Rabbit thymus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's La/SS-B Antigen, Rabbit Thymus can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Researchers should empirically determine the suitability of the La/SS-B Antigen, Rabbit Thymus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "La/SS-B Antigen, Rabbit Thymus, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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