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Testing Data (Natural mixture of streptothricins C (5%), D (51.5%), E (6%) and F (35%) produced by Streptomyces noursei)

Nourseothricin Antibiotic | RB10252 antibiotic

Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT)

Synonyms
Nourseothricin; Nourseothricin (NTC; Nurseotricin; Nourseotricin; clonNAT); RB10252 antibiotic
Ordering
For Research Use Only!
Form/Format
Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.
Application Notes
Suitable for use in selection of recombinant Leishmania strains.
Dilution: Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.
CAS Number
96736-11-7
Components D&F (HPLC)
89.9%
Components D&E (HPLC)
10.2%
Biological Activity
110% standard Nr.82
Ash
2.1% of material.
Elementary Analysis
C: 32.3%
H: 5.7%
N: 14.0%
S: 6.7%
Reconstitution
Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Natural mixture of streptothricins C (5%), D (51.5%), E (6%) and F (35%) produced by Streptomyces noursei)

Testing Data (Natural mixture of streptothricins C (5%), D (51.5%), E (6%) and F (35%) produced by Streptomyces noursei)

Testing Data

(Recombinant green fluorescent L. tarentolae cells (LEXSY) selected with N5375-74)

Testing Data (Recombinant green fluorescent L. tarentolae cells (LEXSY) selected with N5375-74)

Structure

(Structure)

Structure (Structure)
Related Product Information for RB10252 antibiotic
A group of antibiotic substances produced by Streptomyces variants. They are composed of an aminosugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of ß-amino group of the ß-lysine by Nouresothricin N-acetyltransferase the product of the sat1 gene.
Product Categories/Family for RB10252 antibiotic

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1.36kD
NCBI Official Full Name
nourseothricin acetyltransferase
NCBI Official Symbol
RB10252
NCBI Protein Information
nourseothricin acetyltransferase

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Product Notes

The RB10252 (Catalog #AAA651383) is an Antibiotic and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in selection of recombinant Leishmania strains. Dilution: Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the RB10252 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nourseothricin, Antibiotic" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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