Actinomycin D Antibiotic | DHX9 antibiotic

Actinomycin D (Streptomyces antibioticus, Dactinomycin, Actinomycin IV, Actinomycin C1)

Cat. Num. AAA651300

• Gene Names: DHX9; LKP; RHA; DDX9; NDH2; NDHII
• Purity: Cell Culture Grade; 95%; Appearance: Orange to red-brown crystaline powder; Identity: Conforms to USP reference; Potency: 900u/mg; Specific Rotation:-292 to -317º; Heavy Metals: <0.001%; Loss on Drying: 5%; RNase:None Detected; DNas
• Synonyms: Actinomycin D; Actinomycin D (Streptomyces antibioticus; Dactinomycin; Actinomycin IV; Actinomycin C1); DHX9 antibiotic

• Purity/Purification: Cell Culture Grade; 95%; Appearance: Orange to red-brown crystaline powder; Identity: Conforms to USP reference; Potency: 900u/mg; Specific Rotation:-292 to -317º; Heavy Metals: <0.001%; Loss on Drying: 5%; RNase:None Detected; DNas

• CAS Number: 50-76-0
• Molecular Formula: C62H86N12O16
• Preparation and Storage: 4 degree C

Testing Data

(Streptomyces antibioticus; 2-Amino-(N,N)-1-bis(hexadecahydro-6­,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentao­xo-1H-pyrrolo[2,1]-[1,4,7,10,13]oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-ox­o-3H-phenoxazine-1,9-dicarboxamide; Dactinomycin, Actinomycin IV, Actinomycin C1; Actactinomycin A IV; Actinomycin 7; Dilactone Actinomycindioic D Acid; Lyovac Cosmegen; Cosmogen; Meractinomycin)

Related Product Information for DHX9 antibiotic

Suitable for use in nucleic acid inhibition. Actinomycin D inhibits the proliferation of cells in a cell-cycle nonspecific way by forming a stable complex with DNA (via deoxyguanosine residues), thus inhibiting DNA-primed RNA synthesis. It is used in cell culture as a selection agent.

Product Categories/Family for DHX9 antibiotic

Biochemicals; Biochem-Antibiotics

NCBI and Uniprot Product Information

• NCBI GI #: 116241330
• NCBI GeneID: 1660
• UniProt Accession #: Q08211; Q05CI5; Q12803; Q32Q22; Q5VY62; Q6PD69; Q99556; B2RNV4
• Molecular Weight: 1255.43
• NCBI Official Full Name: ATP-dependent RNA helicase A
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 9
• NCBI Official Symbol: DHX9
• NCBI Official Synonym Symbols: LKP; RHA; DDX9; NDH2; NDHII
• NCBI Protein Information: ATP-dependent RNA helicase A; leukophysin; DEAH box protein 9; nuclear DNA helicase II; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9
• UniProt Protein Name: ATP-dependent RNA helicase A
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX9
• UniProt Synonym Gene Names: DDX9; LKP; NDH2; RHA; LKP; NDH II
• UniProt Entry Name: DHX9_HUMAN

NCBI Description

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]

Uniprot Description

Function: Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Positively regulates HIV-1 LTR-directed gene expression. Ref.23 Ref.24 Ref.28

Catalytic activity: ATP + H2O = ADP + phosphate. Ref.30

Subunit structure: Interacts with ZIC2

By similarity. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. Binds MBD2, HRMT1L2/PRMT1, and RELA. May act to directly link BRCA1, CREBBP or SMN1 and the RNA polymerase II complex. Can also interact with XRCC5 and with TOP2A in an RNA dependent manner; these interactions may be indirect. Interaction with TOP2A is promoted by UBC9. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with LARP6. Interacts (via N-terminus) with EIF2AK2/PKR and this interaction is dependent upon the activation of the kinase. Interacts with IGF2BP2 and IGF2BP3. Ref.8 Ref.9 Ref.10 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20 Ref.23 Ref.24 Ref.28 Ref.29

Subcellular location: Nucleus › nucleolus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. Ref.2 Ref.13 Ref.20 Ref.24

Domain: The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. Ref.11 Ref.12

Post-translational modification: Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization. Ref.18May be phosphorylated by PRKDC/XRCC7. Phosphorylated by EIF2AK2/PKR and this phosphorylation perturbs its association with dsRNA. Ref.17 Ref.23

Sequence similarities: Belongs to the DEAD box helicase family. DEAH subfamily.Contains 2 DRBM (double-stranded RNA-binding) domains.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.