Granulocyte Macrophage Colony Stimulating Factor Receptor, alpha Human Active Protein | CSF2RA active protein

Granulocyte Macrophage Colony Stimulating Factor Receptor, alpha, Recombinant Human (GM-CSF Ralpha, CD116)

Cat. Num. AAA651027

• Gene Names: CSF2RA; GMR; CD116; CSF2R; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; MGC3848; MGC4838; GM-CSF-R-alpha
• Reactivity: Human
• Applications: ELISA
• Purity: Purified; 95% by SDS­PAGE under reducing conditions and visualized by silver stain. Endotoxin: 0.01EU per 1ug of the protein by the LAL method.
• Synonyms: Granulocyte Macrophage Colony Stimulating Factor Receptor; alpha Human; alpha; Recombinant Human (GM-CSF Ralpha; CD116); Recombinant Human (GM-CSF R; CSF2RA active protein

• Reactivity: Human
• Purity/Purification: Purified; 95% by SDS­PAGE under reducing conditions and visualized by silver stain. Endotoxin: 0.01EU per 1ug of the protein by the LAL method.
• Form/Format: Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS.

• Applicable Applications for CSF2RA active protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Activity: Measured by its ability to inhibit GMCSFdependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 1.25ug/ml in the presence of 40pg/ml of rhGMCSF.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CSF2RA active protein

Granulocyte macrophage colony stimulating fact or receptor alpha (GM­CSF R), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM­CSF. GM­CSF promotes the differentiation and mobilization of granulocyte­macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effect or functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1 ­ 4). Mature human GM­CSF R is an 80kD type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronect in type III domains and a juxtamembrane WSXWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM­CSF R shares approximately 33% aa sequence identity with mouse and rat GM­CSF R. Alternate splicing of human GM­CSF R generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM­CSF (6, 7). GM­CSF R is expressed on hematopoieticstem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non­hematopoietic solid tumor cells (8). GM­CSF R associates with the common beta chain/CD131 (c), a135kD transmembrane protein that is also the signal transducing component of the receptors for I L­3 and IL­5 (9,10). Association with c convert s GM­CSF R from a low affinity to a high affinity receptor for GM­CSF (9 ­ 11). The shared usage of c underlies the synergism between GM­CSF, I L­3, and I L­5 in their effects on myeloid cell differentiation and activation (1, 2).

Recombinant protein corresponding to human Leu20-Gly320, with a C-terminal 10-His tag expressed in NSO cells (P15509)

Product Categories/Family for CSF2RA active protein

Growth Factors; Cytokines; Growth Factors-GM-CSF

NCBI and Uniprot Product Information

• NCBI GI #: 238908519
• NCBI GeneID: 1438
• NCBI Accession #: NP_001155003.1
• NCBI GenBank Nucleotide #: NM_001161531.1
• UniProt Accession #: P15509; O00207; Q14429; Q14430; Q14431; Q16564; A8KAM1; Q16498
• Molecular Weight: 46,207 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor receptor subunit alpha isoform g
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
• NCBI Official Symbol: CSF2RA
• NCBI Official Synonym Symbols: GMR; CD116; CSF2R; CDw116; CSF2RX; CSF2RY; GMCSFR; CSF2RAX; CSF2RAY; MGC3848; MGC4838; GM-CSF-R-alpha
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor receptor subunit alpha; GMR-alpha; GMCSFR-alpha; CD116 antigen; OTTHUMP00000014504; OTTHUMP00000014505; OTTHUMP00000014506; OTTHUMP00000014507; OTTHUMP00000014508; OTTHUMP00000014917; OTTHUMP00000014918; OTTHUMP00000014919; OTTHUMP00000014920; GM-CSF receptor alpha subunit; colony stimulating factor 2 receptor alpha subunit; granulocyte-macrophage colony-stimulating factor receptor alpha chain
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• Protein Family: Granulocyte-macrophage colony-stimulating factor receptor
• UniProt Gene Name: CSF2RA
• UniProt Synonym Gene Names: CSF2R; CSF2RY
• UniProt Entry Name: CSF2R_HUMAN

NCBI Description

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble. [provided by RefSeq]

Uniprot Description

Function: Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

Subunit structure: Heterodimer of an alpha and a beta subunit. The beta subunit is common to the IL3, IL5 and GM-CSF receptors.

Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform 3: Secreted

Probable. Isoform 4: Secreted

Probable. Isoform 6: Secreted

Probable.

Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation.

Involvement in disease: Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [

MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Ref.11 Ref.12

Miscellaneous: The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.

Sequence similarities: Belongs to the type I cytokine receptor family. Type 5 subfamily.