GRO/KC Active Protein | Cxcl1 active protein
GRO/KC, Recombinant, Mouse (Cxcl1, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, alpha), Cinc1, CINC-1, C-X-C Motif Chemokine 1, Cytokine-induced Neutrophil Chemoattractant 1, Gro1, Growth-regulated alpha Protein, Platelet-derive
Gene Names
Cxcl1; Gro1; CINC-1
Purity
Highly Purified98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Synonyms
GRO/KC; Recombinant; Mouse (Cxcl1; Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity; alpha); Cinc1; CINC-1; C-X-C Motif Chemokine 1; Cytokine-induced Neutrophil Chemoattractant 1; Gro1; Growth-regulated alpha Protein; Platelet-derive; Cxcl1 active protein
Host
E Coli
Purity/Purification
Highly Purified
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
Form/Format
Supplied as a lyophilized powder without additives.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ile-Ala-Asn.
Biological Activity
Mouse CXCL1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-100 ng/ml.
Dimers and Aggregates
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of Mouse KC
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.rietary chromatographic techniques.
1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.rietary chromatographic techniques.
Solubility
It is recommended to reconstitute the lyophilized Recombinant GRO1 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
-20 degree C
Stability: Lyophilized Murine N51/KC although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GRO1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Stability: Lyophilized Murine N51/KC although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GRO1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Related Product Information for Cxcl1 active protein
KC is a member of the CXC chemokine family with homology to human IL-8, a potent neutrophilic chemotactic cytokine. KC is a fundamental mediator of neutrophil recruitment in acute mast cell-dependent skin inflammation.
Recombinant murine KC also nown as N51 and GRO-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7815 Dalton. Recombinant Murine GRO is purified by prop
Recombinant murine KC also nown as N51 and GRO-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7815 Dalton. Recombinant Murine GRO is purified by prop
Product Categories/Family for Cxcl1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
7.82kD
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
Gro1; CINC-1
NCBI Protein Information
growth-regulated alpha protein; C-X-C motif chemokine 1; cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
UniProt Protein Name
Growth-regulated alpha protein
Protein Family
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Cinc1; Gro; Scyb1; CINC-1
UniProt Entry Name
GROA_RAT
NCBI Description
acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]
Uniprot Description
Function: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Subunit structure: Monomer and homodimer.
Subcellular location: Secreted.
Tissue specificity: At least expressed in the lung and trachea.
Induction: By lipopolysaccharides (LPS) and inflammation.
Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Research Articles on Cxcl1
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Product Notes
The Cxcl1 cxcl1 (Catalog #AAA650941) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Il e-Ala-Asn. It is sometimes possible for the material contained within the vial of "GRO/KC, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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