TWEAK Active Protein | TNFSF12 active protein

TWEAK, Recombinant, Human (APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, HCG1991317, MGC129581, MGC20669

Cat. Num. AAA650785

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK
• Purity: > or = 98% by SDS-PAGE and HPLC.
• Synonyms: TWEAK; Recombinant; Human (APO3/DR3 Ligand; APO3L; CD255; DR3LG; FN14; HCG1991317; MGC129581; MGC20669; TNFSF12 active protein

• Purity/Purification: > or = 98% by SDS-PAGE and HPLC.
• Form/Format: Supplied as a lyophilized poder from 10mM sodium phosphate, pH 7.5, 0.1 M L-Arg.
• Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH

• Source: E.coli
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC (peripheral blood mononuclear cells) is less than 10ng/ml. TWEAK wealy induces the death of HT29 cells when cultured in the presence of IFN-y. The ED50 fro this effect if between 30-45 ng/ml
• Country of Origin: USA
• Endotoxin Level: < or = 0.1ng/ug (1EU/ug)
• Reconstitution: Reconstitute with 10mM sodium phosphate pH 7.5 to a concentration of 1 mg/ml. DO NOT VORTEX
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions acan be made in assay buffer.

Related Product Information for TNFSF12 active protein

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. Recombinant Human TWEAK is a 17.0kD protein, comprising the TNF-like extracellular domain of TWEAK.

Product Categories/Family for TNFSF12 active protein

Growth Factors; Cytokines; Growth Factors-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800.1
• NCBI GenBank Nucleotide #: NM_003809.2
• UniProt Accession #: O43508; Q8IZK7; Q8WUZ7
• Molecular Weight: 27,216 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK
• UniProt Entry Name: TNF12_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

Function: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. Ref.7 Ref.10

Subunit structure: Homotrimer

Probable. Interacts with the angiogenic factor AGGF1/VG5Q. Ref.8 Ref.10

Subcellular location: Cell membrane; Single-pass type II membrane protein Ref.3. Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted Ref.3. Isoform TWE-PRIL: Cell membrane; Single-pass membrane protein Ref.3.

Tissue specificity: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.

Sequence similarities: Belongs to the tumor necrosis factor family.

Sequence caution: The sequence AAH19047.1 differs from that shown. Reason: Frameshift at position 125.