Artemin Active Protein | ARTN active protein

Artemin, Recombinant, Human (GFRalpha3-RET Ligand)

Cat. Num. AAA650751

• Gene Names: ARTN; EVN; NBN; ENOVIN
• Purity: Highly Purified; 98% by SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug)
• Synonyms: Artemin; Recombinant; Human (GFRalpha3-RET Ligand); ARTN active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 98% by SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug)
• Form/Format: Supplied as a lyophilized powder from 10mM sodium citrate, pH 4.5, 150mM sodium chloride. Reconstitute with ddH2O, carrier protein to 0.1-0.5mg/ml. DO NOT VORTEX. protein.
• Sequence: AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

• Biological Activity: The ED50 as determined by its ability to stimulate the proliferation of human SH-SY5Y cells is 2.0-5.0ng/ml. The biological activity was also determined by its ability to promote neuronal survival and neurite outgrowth on dorsal root ganglion neurons.
• Country of Origin: USA
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with ddH2O, carrier protein. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ARTN active protein

Artemin is a disulfide-linked homodimeric neurotrophic factor structurally related to GDNF, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. Artemin, GDNF, Persephin and Neurturin all signal through a multicomponent receptor system, composed of RET (receptor tyrosine kinase) and one of the four GFRa (a1-a4) receptors. Artemin prefers the receptor GFRa3-RET, but will use other receptors as an alternative. Artemin supports the survival of all peripheral ganglia such as sympathetic, neural crest and placodally derived sensory neurons, and dopaminergic midbrain neurons. The functional human Artemin ligand is a disulfide-linked homodimer, of two 12.0kD polypeptide monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Recombinant human Artemin is 24.2kD, disulfide-linked homodimer formedby two identical 113 amino acid subunits.

Product Categories/Family for ARTN active protein

Growth Factors; Cytokines; Growth Factors-Artemin

NCBI and Uniprot Product Information

• NCBI GI #: 4235100
• NCBI GeneID: 9048
• UniProt Accession #: Q5T4W7; O95441; O96030; Q6P6A3; D3DPY1; D3DPY3
• Molecular Weight: 24.2kD
• NCBI Official Full Name: Artemin
• NCBI Official Synonym Full Names: artemin
• NCBI Official Symbol: ARTN
• NCBI Official Synonym Symbols: EVN; NBN; ENOVIN
• NCBI Protein Information: artemin; neublastin; OTTHUMP00000009173; OTTHUMP00000231148; OTTHUMP00000231149; OTTHUMP00000231150; OTTHUMP00000231151; OTTHUMP00000231154; neurotrophic factor
• UniProt Protein Name: Artemin
• Protein Family: Artemin
• UniProt Gene Name: ARTN
• UniProt Synonym Gene Names: EVN
• UniProt Entry Name: ARTN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands which are a group of ligands within the TGF-beta superfamily of signaling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenerative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Ref.1 Ref.2

Subunit structure: Homodimer; disulfide-linked

By similarity. Ref.8

Subcellular location: Secreted

Tissue specificity: Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain. Ref.1 Ref.2

Developmental stage: Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain. Ref.1

Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.