Interferon lambda 2 Active Protein | IFNL2 active protein

Interferon lambda 2, Recombinant, Mouse (IFNl2, Interleukin 28A, IL-28A) (BSA Free)

Cat. Num. AAA650700

• Gene Names: IFNL2; IL28A; IL-28A
• Purity: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Interferon lambda 2; Recombinant; Mouse (IFNl2; Interleukin 28A; IL-28A) (BSA Free); IFNL2 active protein

• Host: Recombinant Mouse
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis virus.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IFNL2 active protein

IL-28A (also named interferon-A2, IFN-A2), IL-28B (IFN-A3) and IL-29 (IFN-A1) are type III interferons that are class II cytokine receptor ligands.1-4 They are distantly related to members of the IL-10 family and type I IFN family.1-4 Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species.5 Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene.4 Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases.2-6 They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor b (IL-10 Rb) and IL-28 receptor a (IL-28 Ra; also called IFN-lambda R1).2, 3, 7, 9 Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex.1-3 Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression.2-6 Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses.8 In vivo, however, type III IFNs enhance levels of IFN-y in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system.5, 7 Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs.9-11

A DNA sequence encoding mouse IL-28A (Met 1-Val 193; Accession # Q4VK74) was fused with a six-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 20. kD. As a result of glycosylation, the recombinant mouse IL-28A migrates as an ~ 25-30kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for IFNL2 active protein

Growth Factors; Cytokines; Growth Factors-Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 26024321
• NCBI GeneID: 282616
• NCBI Accession #: NP_742150.1
• NCBI GenBank Nucleotide #: NM_172138.1
• UniProt Accession #: Q8IZJ0; Q6VN55; Q8IWL7
• Molecular Weight: 22,288 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon, lambda 2
• NCBI Official Symbol: IFNL2
• NCBI Official Synonym Symbols: IL28A; IL-28A
• NCBI Protein Information: interferon lambda-2; IFN-lambda-2; interleukin-28A; cytokine Zcyto20; interferon lambda 2; interferon-lambda 2; interleukin 28A (interferon, lambda 2)
• UniProt Protein Name: Interferon lambda-2
• Protein Family: Interferon
• UniProt Gene Name: IFNL2
• UniProt Synonym Gene Names: IL28A; ZCYTO20; IFN-lambda-2; IL-28A
• UniProt Entry Name: IFNL2_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Ref.1 Ref.2 Ref.3

Subcellular location: Secreted Ref.2.

Induction: By viral infections or double-stranded RNA. Ref.1 Ref.2

Sequence similarities: Belongs to the lambda interferon family.

Caution: It is uncertain whether Met-1 or Met-5 is the initiator.

Sequence caution: The sequence AAR24510.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.