Macrophage Derived Chemokine Active Protein | CCL22 active protein

Macrophage Derived Chemokine, Recombinant, Mouse (MDC)

Cat. Num. AAA650601

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; MGC34554; A-152E5.1
• Purity: Highly Purified; ~98% (SDS-PAGE, HPLC)
• Synonyms: Macrophage Derived Chemokine; Recombinant; Mouse (MDC); CCL22 active protein

• Purity/Purification: Highly Purified; ~98% (SDS-PAGE, HPLC)
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile dH2O to 0.1-1mg/ml.

• Biological Activity: The biological activity of MDC was determined by measuring its ability to chemoattract total murine T cells. A concentration range of 10-100ng/ml is found to produce significant chemotaxis. MDC is a member of the b chemokine (C-C) family. MDC is produced by macrophages and monocyte-derived dendritic cells, but is not produced by monocytes, NK cells, or cell lines of epithelial, endothelial, or fibroblast origin.
• Endotoxin Level: ~0.1ng/ug
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for CCL22 active protein

Growth Factors; Cytokines; Growth Factors-MDC

NCBI and Uniprot Product Information

• NCBI GI #: 1931581
• NCBI GeneID: 6367
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 7.8kD, 68 amino acid residues
• NCBI Official Full Name: macrophage-derived chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; MGC34554; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22; MDC(1-69); OTTHUMP00000164658; CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small-inducible cytokine A22; stimulated T cell chemotactic protein 1; stimulated T-cell chemotactic protein 1; small inducible cytokine subfamily A (Cys-Cys), member 22
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22
• UniProt Entry Name: CCL22_HUMAN

NCBI Description

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq]

Uniprot Description

Function: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.

Subcellular location: Secreted.

Tissue specificity: Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. Ref.10

Post-translational modification: The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.