SCF Recombinant Protein

SCF, Recombinant, Human (Stem Cell Factor, Stem Cell Factor Precursor, c-Kit Ligand, DKFZp686F2250, FPH2, KIT Ligand, KitL, KITLG, KL-1, Mast Cell Growth Factor, MGF, SHEP7, Steel Factor, SF)

Cat. Num. AAA650587

• Applications: Western Blot
• Purity: Highly Purified; ~95% (SDS-PAGE, silver stain)
• Synonyms: SCF; Recombinant; Human (Stem Cell Factor; Stem Cell Factor Precursor; c-Kit Ligand; DKFZp686F2250; FPH2; KIT Ligand; KitL; KITLG; KL-1; Mast Cell Growth Factor; MGF; SHEP7; Steel Factor; SF); SCF recombinant protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, silver stain)
• Form/Format: Supplied as lyophilized powder from PBS, 1% HSA, 10% trehalose. Reconstitute with 500ul sterile PBS.

• Applicable Applications for SCF recombinant protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: SCF migrates as a broad band between ~20 and 42kD in SDS-PAGE due to post-translational modifications. Unmodified SCF has a predicted molecular mass of 21.0kD. SCF separates into a number of isoforms with a pI between 3.0 and 7.0 in 2D PAGE due to post-translational modifications. This compares with the unmodified SCF with a predicted pI of 5.76.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for SCF recombinant protein

Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.

Product Categories/Family for SCF recombinant protein

Growth Factors; Cytokines; Growth Factors-Cell Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1246100
• NCBI Official Full Name: stem cell factor
• Protein Family: SCF-associated factor

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; male gonad development; germ cell programmed cell death; signal transduction; cell proliferation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mast cell apoptosis; positive regulation of melanocyte differentiation; innate immune response; positive regulation of Ras protein signal transduction; positive regulation of myeloid leukocyte differentiation; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7