Rabbit anti-Human UBC9 Polyclonal Antibody | anti-UBC9 antibody

UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (MaxLight 490)

Cat. Num. AAA6504417

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: UBC9; Polyclonal Antibody; NT (SUMO-conjugating Enzyme UBC9; SUMO-protein Ligase; Ubiquitin-conjugating Enzyme E2 I; Ubiquitin-protein Ligase I; Ubiquitin Carrier Protein I; Ubiquitin Carrier Protein 9; p18; UBE2I; UBCE9) (MaxLight 490); anti-UBC9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBC9.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-UBC9 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human UBC19, UniProt Accession #P63279.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of A2058 cell line lysates (35ug/lane) usingMBS626003. UBE2I (arrow) was detected using theMBS616215.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue usingMBS626003, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of 293 cell lysates (2ug/lane) using MBS626003 on either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.)

Product Categories/Family for anti-UBC9 antibody

Antibodies; Ubiquitin Ligases

References

1. Biochem Biophys Res Commun. 2002 Aug 30;296(4):870-6. 2. Genomics. 1996 Oct 15;37(2):183-6. 3. Cytogenet Cell Genet. 1996;75(4):222-3. 4. Cytogenet Cell Genet. 1996;72(1):86-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4507785
• NCBI GeneID: 7329
• NCBI Accession #: NP_003336
• NCBI GenBank Nucleotide #: NM_003345.4
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69
• Molecular Weight: 18 kDa (158 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]