Rabbit anti-Human TRIB2 Polyclonal Antibody | anti-TRIB2 antibody

TRIB2, NT (Tribbles Homolog 2, TRB-2, TRB2) (APC)

Cat. Num. AAA6503565

• Gene Names: TRIB2; C5FW; TRB2; GS3955
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TRIB2; Polyclonal Antibody; NT (Tribbles Homolog 2; TRB-2; TRB2) (APC); anti-TRIB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIB2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TRIB2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TRIB2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TRIB2 (arrow) using rabbit polyclonal TRIB2 N-term. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB2 gene (Lane 2).)

Immunohistochemistry (IHC)

(TRIB2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIB2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TRIB2 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TRIB2 antibody

Antibodies; Protein Kinases

References

1. Hegele, R.A., et.al., Hum. Mol. Genet. 18(21), 4189-4194 (2009). 2. Eder, K., et.al., Int. Immunol. 20(12), 1543-1550 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 11056054
• NCBI GeneID: 28951
• NCBI Accession #: NP_067675.1
• NCBI GenBank Nucleotide #: NM_021643.3
• UniProt Accession #: Q92519; B2R851; D6W510
• Molecular Weight: 38,801 Da
• NCBI Official Full Name: tribbles homolog 2
• NCBI Official Synonym Full Names: tribbles pseudokinase 2
• NCBI Official Symbol: TRIB2
• NCBI Official Synonym Symbols: C5FW; TRB2; GS3955
• NCBI Protein Information: tribbles homolog 2
• UniProt Protein Name: Tribbles homolog 2
• Protein Family: Tribbles
• UniProt Gene Name: TRIB2
• UniProt Synonym Gene Names: TRB-2
• UniProt Entry Name: TRIB2_HUMAN

NCBI Description

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

TRB2: Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: cytoplasm; cytoskeleton; nucleus

Molecular Function: mitogen-activated protein kinase kinase binding; nucleotide binding; protein kinase activity; protein kinase inhibitor activity; transcription factor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase regulator activity

Biological Process: negative regulation of fat cell differentiation; negative regulation of interleukin-10 biosynthetic process; negative regulation of protein kinase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of MAP kinase activity