Rabbit anti-Human, Mouse TNIK Polyclonal Antibody | anti-TNIK antibody

TNIK, NT (TRAF2 and NCK-interacting Kinase, KIAA0551) (FITC)

Cat. Num. AAA6503216

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: TNIK; Polyclonal Antibody; NT (TRAF2 and NCK-interacting Kinase; KIAA0551) (FITC); anti-TNIK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNIK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TNIK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TNIK (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-TNIK Pab is used in Western blot to detect TNIK in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(TNIK Antibody (M1) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TNIK antibody detected the TNIK protein (arrow).)

Product Categories/Family for anti-TNIK antibody

Antibodies; Protein Kinases

References

1. Blume-Jensen P, et al. Nature 2001. 411: 355. 2. Cantrell D, J. Cell Sci. 2001. 114: 1439. 3. Jhiang S Oncogene 2000. 19: 5590. 4. Manning G, et al. Science 2002. 298: 1912. 5. Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359. 6.Robertson, S. et al. Trends Genet. 2000. 16: 368. 7.Robinson D, et al. Oncogene 2000. 19: 5548. 8.Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889. 9.Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561. 10.Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

NCBI and Uniprot Product Information

• NCBI GI #: 239735580
• NCBI GeneID: 23043
• NCBI Accession #: NP_001155032.1
• NCBI GenBank Nucleotide #: NM_001161560.1
• UniProt Accession #: Q9UKE5; O60298; Q8WUY7; Q9UKD8; Q9UKD9; Q9UKE0; Q9UKE1; Q9UKE2; Q9UKE3; A7E2A3; A8K4U1; D3DNQ6
• Molecular Weight: 144,251 Da
• NCBI Official Full Name: TRAF2 and NCK-interacting protein kinase isoform 2
• NCBI Official Synonym Full Names: TRAF2 and NCK interacting kinase
• NCBI Official Symbol: TNIK
• NCBI Protein Information: TRAF2 and NCK-interacting protein kinase
• UniProt Protein Name: TRAF2 and NCK-interacting protein kinase
• Protein Family: TRAF2 and NCK-interacting protein kinase
• UniProt Gene Name: TNIK
• UniProt Synonym Gene Names: KIAA0551
• UniProt Entry Name: TNIK_HUMAN

NCBI Description

Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM, Mar 2008]

Uniprot Description

TNIK: Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322. Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1. Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: nucleoplasm; recycling endosome; cytoskeleton; apical plasma membrane; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: microvillus biogenesis; Wnt receptor signaling pathway; protein amino acid autophosphorylation; regulation of dendrite morphogenesis; actin cytoskeleton reorganization; cytoskeleton organization and biogenesis; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; activation of JNKK activity