Bone Morphogenetic Protein 7, Recombinant, Mouse Active Protein | BMP7 active protein
Bone Morphogenetic Protein 7, Recombinant, Mouse (BMP7, OP-1, Osteogenic Protein-1)
Gene Names
BMP7; OP-1
Reactivity
Mouse
Applications
ELISA
Purity
Highly Purified (95% by SDSPAGE under reducing conditions and visualized by silver stain).
Synonyms
Bone Morphogenetic Protein 7; Recombinant; Mouse; Mouse (BMP7; OP-1; Osteogenic Protein-1); BMP7; Osteogenic Protein-1; BMP7 active protein
Host
CHO Cells
Reactivity
Mouse
Specificity
Recognizes mouse BMP-7.
Purity/Purification
Highly Purified (95% by SDSPAGE under reducing conditions and visualized by silver stain).
Form/Format
Lyophilized from a 0.2um filtered solution in HCl. Reconstitute at 100ug/ml in 4mM HCl.
Concentration
~100ug/ml (varies by lot)
Sequence
Ser292
Structure: Disulfide-linked homodimer
Predicted Molecular Mass: 15.6kD (monomer)
SDS-PAGE: 16 20kD under reducing conditions
Structure: Disulfide-linked homodimer
Predicted Molecular Mass: 15.6kD (monomer)
SDS-PAGE: 16 20kD under reducing conditions
Applicable Applications for BMP7 active protein
ELISA
Source Information
Chinese Hamster Ovary cell line, CHO-derived Ser292 His430, Accession# NP_031583
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse
chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is typically 0.125 0.5ug/ml.
chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.
The ED50 for this effect is typically 0.125 0.5ug/ml.
Endotoxin
1.0 EU per 1 ug of the protein by the LAL method.
Preparation and Storage
12 months from date of receipt, 20 to 70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, 20 to 70 degree C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping Temperature: Blue Ice
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, 20 to 70 degree C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping Temperature: Blue Ice
Related Product Information for BMP7 active protein
Bone morphogenetic protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is a widely expressed TGF super family member with important functions during embryogenesis, in the adult, and in disease (1, 2). Mouse BMP7 is synthesized with a 29 amino acid (aa) signal sequence, a 262 aa propeptide, and a 139 aa growth fact or domain (3). The growth factor domain of mouse BMP7 shares 98% and 100% aa sequence identity with human and rat BMP7, respectively. The BMP7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfidelinked growth factor domains (4, 5). Mature BMP7 can also form disulfidelinked heterodimers with BMP2 or BMP4, complexes that show increased potency and range of activity compared to BMP7 homodimers (68). The presence of the propeptides in the BMP7 tetramer does not diminish the bioactivity of the growth factor domains (5). Secreted BMP7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix fibrillin (4). BMP7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPRI I and the t ype 1 receptors Activin RIA, BMPRIA, and BMPRIB (2, 5). BMP7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (9, 10), inhibits the branching of prostate epithelium (11), and antagonizes epithelialmesenchymal transition (EMT) (1214). In pathological conditions, BMP7 inhibits tumor growth and metastasis (13), ameliorates fibrotic damage in nephritis (12), and promotes neuroregeneration following brain ischemia (15).
Product Categories/Family for BMP7 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
15,599 Da
NCBI Official Full Name
bone morphogenetic protein 7
NCBI Official Synonym Full Names
bone morphogenetic protein 7
NCBI Official Symbol
BMP7
NCBI Official Synonym Symbols
OP-1
NCBI Protein Information
bone morphogenetic protein 7; BMP-7; OTTHUMP00000031357; OTTHUMP00000166092; OTTHUMP00000166093; OTTHUMP00000166094; osteogenic protein 1
UniProt Protein Name
Bone morphogenetic protein 7
Protein Family
UniProt Gene Name
BMP7
UniProt Entry Name
Q9H2W7_HUMAN
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Product Notes
The BMP7 bmp7 (Catalog #AAA650269) is an Active Protein produced from CHO Cells and is intended for research purposes only. The product is available for immediate purchase. The Bone Morphogenetic Protein 7, Recombinant, Mouse (BMP7, OP-1, Osteogenic Protein-1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bone Morphogenetic Protein 7, Recombinant, Mouse can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the BMP7 bmp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ser292 Structure: Disulfide- linked homodimer< br>Predict ed Molecular Mass: 15.6kD (monomer)< br>SDS-PAG E: 16 20kD under reducing conditions. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 7, Recombinant, Mouse, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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