DMC Active Protein | DYM active protein

DMC, Recombinant, Human (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)

Cat. Num. AAA650259

• Gene Names: DYM; DMC; SMC
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: DMC; Recombinant; Human (Dendritic cell and Monocyte Chemokine-like Protein; VEGF Co-regulated Chemokine 1; VCC1); DYM active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS, with 50ug of BSA per 1ug of cytokine.

• Activity: Measured by its ability to induce VEGF expression in endothelial cells (Weinstein, E.J. et al., 2006, BBRC, 350:74). The ED50 for this effect is typically 1-5ug/ml.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/ml.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for DYM active protein

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation site. Cleavage of a 23 amino acid (aa) signal sequence yields the mature 96 aa human DMC. DMC is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS-activated peripheral blood monocytes and dendritic cells (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC, plus two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show a correlated expression pattern with VEGF in primary lung, breast and esophageal tumors (2). DMC is, therefore, suggested to play a role in tumor angiogenesis. Mature human DMC shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat DMC, respectively.

Product Categories/Family for DYM active protein

Growth Factors; Cytokines; Growth Factors-Other

NCBI and Uniprot Product Information

• NCBI GI #: 157779137
• NCBI GeneID: 54808
• NCBI Accession #: NP_060123.3
• NCBI GenBank Nucleotide #: NM_017653.3
• UniProt Accession #: Q7RTS9; Q3ZTS8; Q6P2P5; Q8N2M0; Q9BVE9; Q9NPU7; A8K5I8; B2RCF9; B4DKI7
• Molecular Weight: The 96 amino acid residue recombinant human DMC has a predicted molecular mass of approximately 11.3kD.
• NCBI Official Full Name: dymeclin
• NCBI Official Synonym Full Names: dymeclin
• NCBI Official Symbol: DYM
• NCBI Official Synonym Symbols: DMC; SMC
• NCBI Protein Information: dymeclin; dyggve-Melchior-Clausen syndrome protein
• UniProt Protein Name: Dymeclin
• Protein Family: Dymeclin
• UniProt Gene Name: DYM
• UniProt Entry Name: DYM_HUMAN

NCBI Description

This gene encodes a protein which is necessary for normal skeletal development and brain function. Mutations in this gene are associated with two types of recessive osteochondrodysplasia, Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia, which involve both skeletal defects and mental retardation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Necessary for correct organization of Golgi apparatus. Involved in bone development. Ref.8

Subunit structure: Interacts with GOLM1 and PPIB. Ref.8

Subcellular location: Cytoplasm. Golgi apparatus. Note: Sequence analysis programs clearly predict 1 transmembrane region. However, Ref.7 shows that it is not a stably anchored transmembrane protein but it weakly associates with the Golgi apparatus and shuttles between the Golgi and the cytosol. Ref.7 Ref.8

Tissue specificity: Expressed in most embryo-fetal and adult tissues. Abundant in primary chondrocytes, osteoblasts, cerebellum, kidney, lung, stomach, heart, pancreas and fetal brain. Very low or no expression in the spleen, thymus, esophagus, bladder and thyroid gland. Ref.1 Ref.7

Post-translational modification: Myristoylated in vitro; myristoylation is not essential for protein targeting to Golgi compartment.

Involvement in disease: Dyggve-Melchior-Clausen syndrome (DMC) [MIM:223800]: A rare autosomal recessive disorder belonging to the group of spondyloepimetaphyseal dysplasias. DMC is characterized by progressive short stature with short trunk dwarfism, microcephaly, protruding sternum, and psychomotor retardation. Radiological features include a platyspondyly with double vertebral humps, an epiphyso-metaphyseal dysplasia and lacy pelvis iliac crests.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1 Ref.7 Ref.9Smith-McCort dysplasia 1 (SMC1) [MIM:607326]: A rare autosomal recessive osteochondrodysplasia with skeletal features identical to those of Dyggve-Melchior-Clausen syndrome, but with normal intelligence and no microcephaly. It is characterized by short limbs and trunk with barrel-shaped chest. The radiographic phenotype includes platyspondyly, generalized abnormalities of the epiphyses and metaphyses, and a distinctive lacy appearance of the iliac crest.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7 Ref.9 Ref.10

Sequence similarities: Belongs to the dymeclin family.