Rabbit anti-Human TPT1 Polyclonal Antibody | anti-TPT1 antibody

TPT1, ID (Translationally-controlled Tumor Protein, TCTP, p23, Histamine-releasing Factor, HRF, Fortilin) (MaxLight 750)

Cat. Num. AAA6502237

• Gene Names: TPT1; HRF; p02; p23; TCTP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TPT1; Polyclonal Antibody; ID (Translationally-controlled Tumor Protein; TCTP; p23; Histamine-releasing Factor; HRF; Fortilin) (MaxLight 750); anti-TPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TPT1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TPT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human TPT1 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TPT1 Antibody (Center) western blot analysis in LNCaP cell line lysates (35ug/lane).This demonstrates the TPT1 antibody detected the TPT1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TPT1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TPT1 Antibody (Center) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TPT1 antibody

Antibodies; Cancer Markers

References

1. Choi, S., et.al., PLoS ONE 4(6), E5732 (2009). 2. Sanchez, J.C., et.al., Electrophoresis 18(1), 150-155 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4507669
• NCBI GeneID: 7178
• NCBI Accession #: NP_003286
• NCBI GenBank Nucleotide #: NM_003295.3
• UniProt Accession #: P13693; Q6YLS2; Q7Z4J4; Q8TBK7; Q96EE2; Q9UC70; B2R7E5
• Molecular Weight: Predicted: 19 kDa
• NCBI Official Full Name: translationally-controlled tumor protein isoform 2
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: HRF; p02; p23; TCTP
• NCBI Protein Information: translationally-controlled tumor protein; fortilin; histamine-releasing factor
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: TPT1-like protein
• UniProt Gene Name: TPT1
• UniProt Synonym Gene Names: TCTP; HRF
• UniProt Entry Name: TCTP_HUMAN

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Microtubule-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; response to virus; calcium ion transport; negative regulation of apoptosis