Western Blot (WB)
(Western blot analysis of HNF1A Antibody (Center) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse TCF 1 Polyclonal Antibody | anti-TCF1 antibody
TCF 1 (Transcription Factor 1, TCF1, TCF-1, Hepatocyte Nuclear Factor 1-alpha, HNF-1-alpha, HNF1A, HNF-1A, HNF1, IDDM20, Liver-specific Transcription Factor LF-B1, LFB1, MODY3) (APC)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of HNF1A Antibody (Center) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of HNF1A Antibody (Center) in mouse liver tissue lysates (35ug/lane). HNF1A (arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HNF1A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HNF1A Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Uniprot Description
HNF1A: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'- GTTAATNATTAAC-3'. Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF). Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3); also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20). IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Belongs to the HNF1 homeobox family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 12q24.2
Cellular Component: nucleoplasm; protein complex; cytoplasm; nucleus
Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; insulin secretion; glucose import; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis
Disease: Diabetes Mellitus, Insulin-dependent, 20; Diabetes Mellitus, Insulin-dependent; Hepatic Adenomas, Familial; Maturity-onset Diabetes Of The Young, Type 3; Renal Cell Carcinoma, Nonpapillary; Diabetes Mellitus, Noninsulin-dependent
Research Articles on TCF1
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Product Notes
The TCF1 hnf1a (Catalog #AAA6502185) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF 1 (Transcription Factor 1, TCF1, TCF-1, Hepatocyte Nuclear Factor 1-alpha, HNF-1-alpha, HNF1A, HNF-1A, HNF1, IDDM20, Liver-specific Transcription Factor LF-B1, LFB1, MODY3) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TCF 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TCF1 hnf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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