Resistin-like Molecule, beta Recombinant Protein | RETNLB recombinant protein
Resistin-like Molecule, beta, Recombinant, Human (RELMb)
Gene Names
RETNLB; XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
Purity
Highly Purified95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Synonyms
Resistin-like Molecule; beta; Recombinant; Human (RELMb); RETNLB recombinant protein
Host
E Coli
Specificity
The amino acid sequence of the recombinant human RELM-beta is 100% homologous to the amino acid sequence of the human RELM-beta without signal sequence.
Purity/Purification
Highly Purified
95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Form/Format
Supplied as a lyophilized powder in 0.05M acetate buffer, pH 4.0.
Sequence
MG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQLETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH.
Application Notes
Western blotting, ELISA
Solubility
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.
Preparation and Storage
-20 degree C
Stability: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C. The lyophilized protein remains stable until the expiry date when stored at -20 degree C.
Stability: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C. The lyophilized protein remains stable until the expiry date when stored at -20 degree C.
Related Product Information for RETNLB recombinant protein
RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. RELM-beta appears to be produced as a homodimer exclusively by intestinal goblet cells and can be found in high quantities in stool. Remarkably, stool of germ-free mice displaying sterile intestinal tract does not contain RELM-beta until bacterial colonization takes place after pathogen-free mice entered natural environment. Some, but not all, colon carcinoma cell lines secrete RELM-beta into the cell culture supernatant. The physiological function of RELM-beta is not known. High doses of recombinant RELM-beta showed hyperglycemic effects including lowered glucose disposal and increased hepatic glucose production in mice.
Recombinant Human RELM-beta is a His-Tagged Fusion Protein, 11kD protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues-HisTag (underlined).
Recombinant Human RELM-beta is a His-Tagged Fusion Protein, 11kD protein containing 90 amino acid residues of the human RELM-beta and 12 additional amino acid residues-HisTag (underlined).
Product Categories/Family for RETNLB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11kD
NCBI Official Full Name
resistin-like beta
NCBI Official Synonym Full Names
resistin like beta
NCBI Official Symbol
RETNLB
NCBI Official Synonym Symbols
XCP2; FIZZ1; FIZZ2; HXCP2; RELMb; RELMbeta; RELM-beta
NCBI Protein Information
resistin-like beta; found in inflammatory zone 1; colon carcinoma-related gene protein; cysteine-rich secreted protein FIZZ2; cysteine-rich secreted A12-alpha-like protein 1; cysteine-rich secreted protein A12-alpha-like 1; colon and small intestine-specific cysteine-rich protein; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2
UniProt Protein Name
Resistin-like beta
Protein Family
UniProt Gene Name
RETNLB
UniProt Synonym Gene Names
CCRG; FIZZ2; HXCP2; RETNL2
UniProt Entry Name
RETNB_HUMAN
Uniprot Description
Function: Probable hormone.
Subunit structure: Homodimer; disulfide-linked
By similarity.
Subcellular location: Secreted.
Tissue specificity: Expressed only in the gastrointestinal tract, particularly the colon.
Sequence similarities: Belongs to the resistin/FIZZ family.
Research Articles on RETNLB
Similar Products
Product Notes
The RETNLB retnlb (Catalog #AAA650108) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Western blotting, ELISA. Researchers should empirically determine the suitability of the RETNLB retnlb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MG STQCSLDS VMDKKIKDVL NSLEYSPSPI SKKLSCASVK SQGRPSSCPA GMAVTGCACG YGCGSWDVQL ETTCHCQCSV VDWTTARCCH LTKLRSHHHH HH. It is sometimes possible for the material contained within the vial of "Resistin-like Molecule, beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
