Rabbit anti-Human SNURF Polyclonal Antibody | anti-SNURF antibody

SNURF, ID (SNRPN Upstream Reading Frame Protein) (MaxLight 750)

Cat. Num. AAA6500071

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SNURF; Polyclonal Antibody; ID (SNRPN Upstream Reading Frame Protein) (MaxLight 750); anti-SNURF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNURF.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
• Sequence Length: 71

• Applicable Applications for anti-SNURF antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SNURF (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SNURF Antibody (Center) in Hela cell line lysates (35ug/lane). SNURF(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB8A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SNURF Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SNURF antibody

Antibodies; Proteins

References

1. Rodriguez-Jato, S., Nucleic Acids Res. 33(15), 4740-4753 (2005). 2. Runte, M., Hum. Genet. 114(6), 553-561 (2004). 3. Runte, M., Hum. Mol. Genet. 10(23), 2687-2700 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13027653
• NCBI GeneID: 8926
• NCBI Accession #: NP_005669
• NCBI GenBank Nucleotide #: NP_005669.2
• UniProt Accession #: Q9Y675
• Molecular Weight: 841210.58
• NCBI Official Full Name: SNRPN upstream reading frame protein
• NCBI Official Synonym Full Names: SNRPN upstream reading frame
• NCBI Official Symbol: SNURF
• NCBI Protein Information: SNRPN upstream reading frame protein
• UniProt Protein Name: SNRPN upstream reading frame protein
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNURF
• UniProt Entry Name: SNURF_HUMAN

NCBI Description

This gene is located within the Prader-Willi Syndrome critical region on chromosome 15. Transcripts produced from this gene initiate at an imprinting center and are paternally-imprinted. These transcripts may be bicistronic and also encode SNRPN (small nuclear ribonucleoprotein polypeptide N) from a downstream open reading frame. The small protein represented by this gene is encoded by an evolutionarily-conserved upstream open reading frame and is localized to the nucleus. Extensive alternative splicing and promoter usage occurs in this region and the full-length nature of some of these transcripts has not been determined. Alterations in the imprinting center are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]

Uniprot Description

SNURF: a highly basic 71-aa nuclear protein. Encoded with SNRPN on a polycistronic transcript. Play an important role in maintaining differentialy methylated DNA. The gene is imprinted, and disruption of imprinting plays a role in Prader-Willi syndrome (PWS). Translated in normal, but not PWS, human, and mouse tissues.

Protein type: Transcription regulation; Gene silencing

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: nucleus