Western Blot (WB)
(NGRN Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OR52D1 antibody detected the NGRN protein (arrow).)
Rabbit anti-Human NGRN Polyclonal Antibody | anti-NGRN antibody
NGRN, CT (NGRN, FI58G, Neugrin, Mesenchymal stem cell protein DSC92, Neurite outgrowth-associated protein, Spinal cord-derived protein FI58G)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(NGRN Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OR52D1 antibody detected the NGRN protein (arrow).)
Western Blot (WB)
(NGRN Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the OR52D1 antibody detected the NGRN protein (arrow).)
Immunohistochemistry (IHC)
(NGRN antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGRN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NGRN antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NGRN antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(NGRN Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(NGRN Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
Function: May be involved in neuronal differentiation.
Subcellular location: Nucleus. Secreted
Potential Ref.1.
Tissue specificity: Expressed at high levels in heart, brain and skeletal muscle. In brain, mainly expressed in neurons rather than glial cells. Ref.1
Induction: Highly up-regulated in neuroblastostoma cells by retinoic acid treatment inducing neurite outgrowth.
Sequence similarities: Belongs to the neugrin family.
Sequence caution: The sequence AAF65447.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence AAG09725.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence AAG09725.1 differs from that shown. Reason: Frameshift at position 47. The sequence AAH01682.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence AAH07222.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence AAH09389.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence AAH17192.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence BAB21533.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence BAB21533.1 differs from that shown. Reason: Frameshift at position 47. The sequence BAG35525.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence BAG35525.1 differs from that shown. Reason: N-terminus does not match isoform 2.The sequence CAB96088.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence CAD39160.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.
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Product Notes
The NGRN ngrn (Catalog #AAA649928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NGRN, CT (NGRN, FI58G, Neugrin, Mesenchymal stem cell protein DSC92, Neurite outgrowth-associated protein, Spinal cord-derived protein FI58G) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NGRN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the NGRN ngrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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