Rabbit anti-Human SGK2 Polyclonal Antibody | anti-SGK2 antibody

SGK2, CT (Serine/Threonine-protein Kinase Sgk2, Serum/Glucocorticoid Regulated Kinase 2) (AP)

Cat. Num. AAA6499132

• Gene Names: SGK2; H-SGK2; dJ138B7.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SGK2; Polyclonal Antibody; CT (Serine/Threonine-protein Kinase Sgk2; Serum/Glucocorticoid Regulated Kinase 2) (AP); anti-SGK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SGK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SGK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SGK2 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SKG2 Pab in placenta lysate. SKG2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-SGK2 antibody

Antibodies; Protein Kinases

References

1. Friedrich, B., et al., Pflugers Arch. 445(6):693-696 (2003). 2. Embark, H.M., et al., Pflugers Arch. 445(5):601-606 (2003). 3. Gamper, N., et al., Pflugers Arch. 445(1):60-66 (2002). 4. Lang, F., et al., Sci. STKE 2001 (108), RE17 (2001). 5. Kobayashi, T., et al., Biochem. J. 344 Pt 1, 189-197 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 20127541
• NCBI GeneID: 10110
• NCBI Accession #: NP_057360.2
• NCBI GenBank Nucleotide #: NM_016276.3
• UniProt Accession #: Q9HBY8; Q52PK5; Q5H8Y6; Q5H8Z1; Q5TZR3; Q9UKG6
• Molecular Weight: 43,977 Da
• NCBI Official Full Name: serine/threonine-protein kinase Sgk2 isoform beta
• NCBI Official Synonym Full Names: serum/glucocorticoid regulated kinase 2
• NCBI Official Symbol: SGK2
• NCBI Official Synonym Symbols: H-SGK2; dJ138B7.2
• NCBI Protein Information: serine/threonine-protein kinase Sgk2
• UniProt Protein Name: Serine/threonine-protein kinase Sgk2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SGK2
• UniProt Entry Name: SGK2_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

SGK2: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, survival and proliferation. Up- regulates Na(+) channels: SCNN1A/ENAC, K(+) channels: KCNA3/Kv1.3, KCNE1 and KCNQ1, amino acid transporter: SLC6A19, glutamate transporter: SLC1A6/EAAT4, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Highly expressed in liver, kidney and pancreas, and at lower levels in brain. Two specific sites, one in the kinase domain (Thr-253) and the other in the C-terminal regulatory region (Ser- 416), need to be phsphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; SGK family

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; potassium channel regulator activity; sodium channel regulator activity; ATP binding

Biological Process: positive regulation of transporter activity; regulation of cell growth; response to oxidative stress; transmembrane transport; protein amino acid phosphorylation; regulation of cell proliferation