Rabbit SARS Polyclonal Antibody | anti-SARS antibody

SARS, Spike Protein (CT) (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus) (Biotin)

Cat. Num. AAA6498473

• Gene Names: S; E2
• Applications: ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SARS; Polyclonal Antibody; Spike Protein (CT) (Severe Acute Respiratory Syndrome; SARS-CoV; SARS Associated Coronavirus) (Biotin); anti-SARS antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the C-terminal of the spike protein of SARS-associated coronavirus.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.

• Applicable Applications for anti-SARS antibody: ELISA (EIA)
• Application Notes: ELISA: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of the SARS Spike glycoprotein.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-SARS antibody

Antibodies; Infectious Disease SARS

References

1. Marra, MA., et al., (2003), "The Genome sequence of the SARS-associated corona virus", Science, 300, 1399-1404. 2. Rota, P.A., et al., (2003), "Characterization of a novel coronavirus associated with severe acute respiratory syndrome", Science, 300, 1394-1399. 3. Navas-Martin, S.R., et al., (2004), Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development", J. Neurovirol., 10, 75-85. 4. Li, W., et al., (2003). "Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus", Nature, 426, 450-454.

NCBI and Uniprot Product Information

• NCBI GI #: 30173397
• NCBI GeneID: 1489668
• UniProt Accession #: P59594; Q6QU82; Q7T696; Q7TA19; Q7TFA2; Q7TFB1; Q80BV6
• Molecular Weight: 139,125 Da
• NCBI Official Full Name: Spike glycoprotein
• NCBI Official Symbol: S
• NCBI Official Synonym Symbols: E2
• NCBI Protein Information: E2 glycoprotein precursor; spike glycoprotein
• UniProt Protein Name: Spike glycoprotein
• Protein Family: Serine--tRNA ligase
• UniProt Gene Name: S
• UniProt Synonym Gene Names: S glycoprotein
• UniProt Entry Name: SPIKE_CVHSA

Uniprot Description

S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.