Rabbit anti-Human RSK2 Polyclonal Antibody | anti-RSK2 antibody

RSK2, NT (Ribosomal S6 Kinase 2, RSK2, RSK-2, p90-RSK2, pp90RSK2, 90kD Ribosomal Protein S6 Kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated Protein Kinase 1, ISPK1, ISPK-1, MAP Kinase-activated Protein Kinase 1b, MAPK-activated Protein Kinase 1b, MAPKAP

Cat. Num. AAA6498286

• Gene Names: RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RSK2; Polyclonal Antibody; NT (Ribosomal S6 Kinase 2; RSK-2; p90-RSK2; pp90RSK2; 90kD Ribosomal Protein S6 Kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated Protein Kinase 1; ISPK1; ISPK-1; MAP Kinase-activated Protein Kinase 1b; MAPK-activated Protein Kinase 1b; MAPKAP; Ribosomal Protein S6 Kinase 90kDa Polypeptide 3; CLS; EC=2.7.11.1; HU-3; MRX19; RSK; anti-RSK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RSK3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RSK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RSK3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RSK3 (arrow) using rabbit polyclonal RSK3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KA3 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of anti-RSK3 Antibody (N-term) in Ramos cell line lysates (35ug/lane). RSK3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with RSK3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-RSK2 antibody

Antibodies; Protein Kinases; Ribosomal

References

1. Yang, X., et al., Cell 117(3):387-398 (2004). 2. Guimiot, F., et al., Gene Expr. Patterns 4(1):111-114 (2004). 3. Zeniou, M., et al., (er) Nucleic Acids Res. 32(3):1214-1223 (2004). 4. Vaidyanathan, H., et al., J. Biol. Chem. 278(34):32367-32372 (2003). 5. Zhang, Y., et al., J. Biol. Chem. 278(15):12650-12659 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4759050
• NCBI GeneID: 6197
• NCBI Accession #: NP_004577
• NCBI GenBank Nucleotide #: NM_004586.2
• UniProt Accession #: P51812; Q4VAP3; Q59H26; Q5JPK8; Q7Z3Z7; B2R9V4
• Molecular Weight: 83,736 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• NCBI Official Symbol: RPS6KA3
• NCBI Official Synonym Symbols: CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• UniProt Gene Name: RPS6KA3
• UniProt Synonym Gene Names: ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
• UniProt Entry Name: KS6A3_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]