Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Rabbit anti-Human PET112L Polyclonal Antibody | anti-PET112 antibody
PET112L, NT (PET112, PET112L, Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial, Cytochrome oxidase assembly factor PET112 homolog)
Gene Names
PET112; HSPC199; PET112L
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
PET112L; Polyclonal Antibody; NT (PET112; Glutamyl-tRNA(Gln) amidotransferase subunit B; mitochondrial; Cytochrome oxidase assembly factor PET112 homolog); Anti -PET112L; anti-PET112 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-PET112 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
PET112L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human PET112L.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Western Blot (WB)
(PET112L Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PET112L antibody detected the PET112L protein (arrow).)
Related Product Information for anti-PET112 antibody
PET112L furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (Potential).
Product Categories/Family for anti-PET112 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,864 Da
NCBI Official Full Name
glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial isoform X1
NCBI Official Synonym Full Names
PET112 homolog (yeast)
NCBI Official Symbol
PET112
NCBI Official Synonym Symbols
HSPC199; PET112L
NCBI Protein Information
glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; glu-ADT subunit B; cytochrome oxidase assembly factor PET112 homolog; cytochrome c oxidase assembly factor PET112 homolog
UniProt Protein Name
Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
UniProt Gene Name
PET112
UniProt Synonym Gene Names
PET112L; Glu-AdT subunit B
UniProt Entry Name
GATB_HUMAN
Uniprot Description
Function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). Ref.9
Catalytic activity: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_03147
Subunit structure: Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits.
Subcellular location: Mitochondrion Ref.1.
Tissue specificity: Predominantly expressed in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle and heart. Ref.1
Sequence similarities: Belongs to the GatB/GatE family. GatB subfamily.
Sequence caution: The sequence AAF36119.1 differs from that shown. Reason: Frameshift at position 420.
Research Articles on PET112
Similar Products
Product Notes
The PET112 pet112 (Catalog #AAA649731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PET112L, NT (PET112, PET112L, Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial, Cytochrome oxidase assembly factor PET112 homolog) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PET112L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PET112 pet112 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PET112L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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